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盐酸小檗碱对中华绒螯蟹免疫反应的影响。

Effects of berberine hydrochloride on immune response in the crab Charybdis japonica.

机构信息

Institute of Marine Biology, College of Oceanography, Hohai University, Nanjing, 210024, China.

Biology Program, School of Distance Education, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia.

出版信息

BMC Genomics. 2022 Aug 11;23(1):578. doi: 10.1186/s12864-022-08798-w.

DOI:10.1186/s12864-022-08798-w
PMID:35953779
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9373360/
Abstract

Berberine hydrochloride is the main effective component of Coptis spp. used in Chinese herbal medicine and its underlying molecular mechanisms, responsible for inducing effects in crustacean species, are not fully understood. In this study, the molecular response of the crab Charybdis japonica to berberine hydrochloride exposure was studied using transcriptome sequencing. The survival rate, gene expression and activities of several immune enzymes were measured after berberine hydrochloride treatments, with or without injection of the pathogenic bacterium Aeromonas hydrophila. A total of 962 differentially expressed genes (464 up-regulated and 498 down-regulated) were observed during exposure to 100 mg/L of berberine hydrochloride and in the control group after 48 h. Enrichment analysis revealed that these genes are involved in metabolism, cellular processes, signal transduction and immune functions, indicating that exposure to berberine hydrochloride activated the immune complement system. This bioactive compound simultaneously activated fibrinogen beta (FGB), fibrinogen alpha (FGA), alpha-2-macroglobulin (A2M), kininogen (KNG), fibrinogen gamma chain (FGB), alpha-2-HS-glycoprotein (AHSG), caspase-8 (CASP8), cathepsin L (CTSL), adenylate cyclase 3 (Adcy3) and MMP1. Its action could significantly increase the survival rate of the crabs injected with A. hydrophila and promote the activity of LZM, Caspas8, FGA, ACP and AKP in the hepatopancreas. When A. hydrophila was added, the neutralization of 300 mg/L berberine hydrochloride maximized the activities of Caspas8, LZM, ACP and AKP. Our results provide a new understanding of the potential effects of berberine hydrochloride on the immune system mechanisms in crustaceans.

摘要

盐酸小檗碱是中草药黄连的主要有效成分，其在甲壳动物物种中诱导作用的潜在分子机制尚不完全清楚。在这项研究中，使用转录组测序研究了日本绒螯蟹对盐酸小檗碱暴露的分子反应。在盐酸小檗碱处理后，测量了存活率、基因表达和几种免疫酶的活性，同时还注射了致病性细菌嗜水气单胞菌。在暴露于 100mg/L 盐酸小檗碱和对照组 48 小时后，观察到总共 962 个差异表达基因（464 个上调和 498 个下调）。富集分析表明，这些基因参与代谢、细胞过程、信号转导和免疫功能，表明暴露于盐酸小檗碱激活了免疫补体系统。这种生物活性化合物同时激活了纤维蛋白原β（FGB）、纤维蛋白原α（FGA）、α-2-巨球蛋白（A2M）、激肽原（KNG）、纤维蛋白原γ链（FGB）、α-2-HS-糖蛋白（AHSG）、半胱天冬酶 8（CASP8）、组织蛋白酶 L（CTSL）、腺苷酸环化酶 3（Adcy3）和 MMP1。它的作用可以显著提高注射嗜水气单胞菌的螃蟹的存活率，并促进肝胰腺中 LZM、Caspas8、FGA、ACP 和 AKP 的活性。当添加嗜水气单胞菌时，300mg/L 盐酸小檗碱的中和作用使 Caspas8、LZM、ACP 和 AKP 的活性最大化。我们的研究结果为盐酸小檗碱对甲壳动物免疫系统机制的潜在影响提供了新的认识。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6989/9373360/18a19be33845/12864_2022_8798_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6989/9373360/fee357115f46/12864_2022_8798_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6989/9373360/c8c52dbbb121/12864_2022_8798_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6989/9373360/777e98f3f7f0/12864_2022_8798_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6989/9373360/1f3bf77c9f63/12864_2022_8798_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6989/9373360/ddf51692e5b4/12864_2022_8798_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6989/9373360/aec317f8c2e2/12864_2022_8798_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6989/9373360/18a19be33845/12864_2022_8798_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6989/9373360/fee357115f46/12864_2022_8798_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6989/9373360/c8c52dbbb121/12864_2022_8798_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6989/9373360/777e98f3f7f0/12864_2022_8798_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6989/9373360/1f3bf77c9f63/12864_2022_8798_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6989/9373360/ddf51692e5b4/12864_2022_8798_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6989/9373360/aec317f8c2e2/12864_2022_8798_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6989/9373360/18a19be33845/12864_2022_8798_Fig7_HTML.jpg

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