East China Normal University, Shanghai, 200241, China.
East China Normal University, Shanghai, 200241, China; Department of Aquaculture, College of Marine Sciences, Hainan University, Haikou, Hainan, 570228, China.
Aquat Toxicol. 2018 Oct;203:150-158. doi: 10.1016/j.aquatox.2018.08.014. Epub 2018 Aug 18.
Benzo(a)pyrene (BaP) is a highly toxic polycyclic aromatic hydrocarbon and has strong affinity to suspended materials and sediments in the aquatic environment. Most crustaceans are benthic species and are easily affected by the pollution in the sediments, but there is little information on the response mechanism of crustaceans to BaP exposure. This study compared the growth and hepatopancreas transcriptomic responses of the Chinse mitten crab (Eriocheir sinensis) exposed to 0, 0.15 (BaP1) and 0.45 μg /L (BaP2) for 28 days. Crab survival and weight gain were reduced in the water born BaP in a dose-dependent way. The contents of hepatopancreas glycogen, triglyceride, total amino acids and lactic acid were all decreased after BaP exposure, indicating possible more energy consumption during detoxification. In the transcriptome analysis, a total of 106.65 million clean reads were obtained and assembled into 81,714 unigenes with an average length of 594 bp and N50 of 808 bp. Under 0.15 or 0.45 μg /L BaP exposure, 922 and 1129 unigenes in crabs were significantly expressed, annotated to 676 and 802 Gene Ontology (GO) terms respectively. The "cellular process" was the leading category for both concentrations. Thirteen significantly changed pathways were identified in both Control vs BaP1 and Control vs BaP2 groups. These pathways were divided into four different parts according to their reported functions, including metabolism, environmental information processing, organismal systems and cellular processes. Nice out of thirteen pathways in BaP1 were related to metabolism, containing amino acid metabolism, phenylpropanoid biosynthesis, monobactam biosynthesis and styrene degradation. Almost all the pathways related with the biosynthesis processes were down-regulated, while the degradation pathways were up-regulated. Seven out of thirteen pathways were classified into metabolism category in BaP2. These pathways were mostly associated with stress resistance rather than supplying energy. This study indicates that both concentrations of BaP disturbed nutrient metabolism, immune response and defense system in the crabs, while exposure to a higher concentration had a greater impact on immunity system than on metabolism. This study provides a better understanding of the underlying molecular and regulatory mechanisms in crustaceans coping with BaP toxicity.
苯并(a)芘（BaP）是一种高度毒性的多环芳烃，对水生态系统中的悬浮物质和沉积物具有很强的亲和力。大多数甲壳类动物是底栖物种，容易受到沉积物污染的影响，但关于甲壳类动物对 BaP 暴露的反应机制的信息很少。本研究比较了中华绒螯蟹（Eriocheir sinensis）在暴露于 0、0.15（BaP1）和 0.45μg/L（BaP2）28 天后的生长和肝胰腺转录组反应。水中 BaP 呈剂量依赖性地降低了蟹的存活率和体重增加。暴露于 BaP 后，肝胰腺糖原、甘油三酯、总氨基酸和乳酸含量均降低，表明解毒过程中可能消耗更多能量。在转录组分析中，共获得 10665 万条清洁读数，组装成 81714 条 unigenes，平均长度为 594bp，N50 为 808bp。在 0.15 或 0.45μg/L BaP 暴露下，蟹体内有 922 和 1129 个 unigenes显著表达，分别注释到 676 和 802 个基因本体论（GO）术语。对于这两种浓度，“细胞过程”都是主要类别。在对照与 BaP1 组和对照与 BaP2 组之间，共鉴定出 13 个显著变化的途径。根据其报道的功能，这些途径分为代谢、环境信息处理、生物体系统和细胞过程四个不同部分。BaP1 中 13 个途径中的 7 个与代谢有关，包括氨基酸代谢、苯丙素生物合成、单胞菌素生物合成和苯乙烯降解。与生物合成过程相关的几乎所有途径都下调，而降解途径上调。BaP2 中 13 个途径中的 7 个被归类为代谢类别。这些途径主要与应激反应有关，而不是提供能量。本研究表明，两种浓度的 BaP 均干扰了蟹的营养代谢、免疫反应和防御系统，而暴露于较高浓度对免疫系统的影响大于对代谢的影响。本研究为了解甲壳类动物应对 BaP 毒性的潜在分子和调控机制提供了更好的认识。
