Zhang Zhian, Li Fei, Ma Xiaowen, Li Fadi, Wang Zongli
State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China.
Animals (Basel). 2022 Jul 30;12(15):1941. doi: 10.3390/ani12151941.
This study aimed to explore the effects of different levels of barley starch instead of corn starch on the rumen fermentation and microflora when feeding a corn-based diet to Hu sheep. Thirty-two male Hu sheep equipped with permanent rumen fistulas were selected and fed in individual metabolic cages. All sheep were randomly divided into four groups (eight sheep in each group) and fed with four diets containing a similar starch content, but from different starch sources, including 100% of starch derived from corn (CS), 33% of starch derived from barley + 67% of starch derived from corn (33 BS), 67% of starch derived from barley + 33% of starch derived from corn (67 BS) and 100% of starch derived from barley (100 BS). The experimental period included a 14 d adaptation period and a 2 d continuous data collection period. The results showed that the molar proportions of acetate, isobutyrate, butyrate and isovalerate and the ratio of acetate to propionate in the 67 BS and 100 BS groups decreased compared with the CS and 33 BS groups (p < 0.001), while the molar proportions of propionate and valerate increased (p < 0.001). The combination of 33% barley starch and 67% corn starch in the diet improved the production of TVFAs (p = 0.007). The OTUs and Shannon indexes of the CS and 33 BS groups were higher than the 67 BS and 100 BS groups (p < 0.001), and the Chao1 and Ace indexes were higher than the 100 BS group (p < 0.05). In addition, the 33 BS group had increased the relative abundances of Bacteroidetes, Prevotella and Ruminococcus and the abundances of Fibrobacter succinogenes, Ruminococcus flavefaciens, Streptococcus bovis, Selenomonas ruminantium and Prevotella brevis relative to the CS group (p < 0.05). These results indicate that the substitution of 33% of the CS with BS did not change the rumen fermentation pattern relative to the CS group, and increased the richness and diversity of the rumen microbes in Hu sheep compared with other two starch substitute groups.
本研究旨在探讨在给湖羊饲喂以玉米为基础的日粮时，用不同水平的大麦淀粉替代玉米淀粉对瘤胃发酵和微生物区系的影响。选取32只装有永久性瘤胃瘘管的雄性湖羊，将其饲养在个体代谢笼中。所有绵羊随机分为四组（每组8只），分别饲喂四种淀粉含量相似但来源不同的日粮，包括100%玉米来源的淀粉（CS）、33%大麦来源的淀粉 + 67%玉米来源的淀粉（33 BS）、67%大麦来源的淀粉 + 33%玉米来源的淀粉（67 BS）和100%大麦来源的淀粉（100 BS）。试验期包括14天的适应期和2天的连续数据收集期。结果表明，与CS组和33 BS组相比，67 BS组和100 BS组中乙酸、异丁酸、丁酸和异戊酸的摩尔比例以及乙酸与丙酸的比值均降低（p < 0.001），而丙酸和戊酸的摩尔比例升高（p < 0.001）。日粮中33%的大麦淀粉与67%的玉米淀粉组合提高了总挥发性脂肪酸的产量（p = 0.007）。CS组和33 BS组的操作分类单元（OTUs）和香农指数高于67 BS组和100 BS组（p < 0.001），Chao1指数和Ace指数高于100 BS组（p < 0.05）。此外，相对于CS组，33 BS组中拟杆菌门、普雷沃氏菌属和瘤胃球菌属的相对丰度以及琥珀酸纤维杆菌、黄化瘤胃球菌、牛链球菌、反刍月形单胞菌和短普雷沃氏菌的丰度增加（p < 0.05）。这些结果表明，用33%的大麦淀粉替代玉米淀粉相对于CS组并未改变瘤胃发酵模式，并且与其他两个淀粉替代组相比，增加了湖羊瘤胃微生物的丰富度和多样性。
