Pornpanom Pornchai, Kasorndorkbua Chaiyan, Lertwatcharasalakul Preeda, Salakij Chaleow
Akkhararatchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand.
Department of Pathology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
Animals (Basel). 2022 Aug 5;12(15):1988. doi: 10.3390/ani12151988.
In attempt to treat injured raptors and promote conservation awareness, the Kasetsart University Raptor Rehabilitation Unit (KURRU) was established in 2007. The complete blood counts (CBCs) are a manual tool used for the screening of raptor health. These tests require knowledge of blood cell morphology. This study aimed to describe the preliminary information of the hematology, ultrastructure, and morphology of blood cells in rufous-winged buzzards (RWB). There were 17 RWBs admitted into the KURRU. CBCs were manually performed by veterinary technicians. The morphology and morphometry of blood cells were observed from Wright-stained blood smears. Ultrastructure was observed from uranyl acetate and lead citrate-stained sections. The hematologic values were analyzed and described from individual RWBs that were clinically healthy, negative for blood parasites, and had PCV > 0.30 L/L. Consequently, CBCs of 12 out of 17 RWBs were included for descriptive hematologic values. Heterophils were the most prevalent white blood cells in RWBs. Of these 17 RWBs, 1 non-parasitized RWB showed hypochromic erythrocytes with PCV 0.18 L/L, which indicated that anemia in RWBs resulted from non-parasitic causes. The morphology of blood cells in RWBs was similar to those in other diurnal raptors, except that the lymphocytes showed pale or colorless cytoplasm. The electron micrographs highlighted that the basophil contained two types of granules: homogeneous electron-dense granules and reticulated electron-dense granules. The photomicrographs in this report are the scientific reference for identification of blood cells in RWBs. The CBCs from non-parasitized RWBs (clinically healthy) can be used as a cage mate reference in the KURRU. Additionally, we found evidence that evaluations of blood smears together with CBC examination were important in raptors.
为了治疗受伤的猛禽并提高保护意识，2007年成立了 Kasetsart 大学猛禽康复单位（KURRU）。全血细胞计数（CBC）是用于筛查猛禽健康状况的一种手工工具。这些检测需要血细胞形态学知识。本研究旨在描述棕翅鵟（RWB）血细胞的血液学、超微结构和形态的初步信息。有17只棕翅鵟被收治到 KURRU。全血细胞计数由兽医技术人员手动进行。从经 Wright 染色的血涂片观察血细胞的形态和形态测量。从经醋酸铀和柠檬酸铅染色的切片观察超微结构。对临床健康、血液寄生虫检测呈阴性且红细胞压积>0.30 L/L 的个体棕翅鵟的血液学值进行分析和描述。因此，17只棕翅鵟中有12只的全血细胞计数被纳入描述性血液学值分析。嗜异性粒细胞是棕翅鵟中最常见的白细胞。在这17只棕翅鵟中，1只未感染寄生虫的棕翅鵟红细胞压积为0.18 L/L，表现为低色素性红细胞，这表明棕翅鵟的贫血是由非寄生虫原因引起的。棕翅鵟血细胞的形态与其他昼行性猛禽相似，只是淋巴细胞的细胞质呈淡色或无色。电子显微镜照片显示嗜碱性粒细胞含有两种类型的颗粒：均匀的电子致密颗粒和网状电子致密颗粒。本报告中的显微照片是鉴定棕翅鵟血细胞的科学参考。未感染寄生虫（临床健康）的棕翅鵟的全血细胞计数可作为 KURRU 中笼养同伴的参考。此外，我们发现有证据表明，血液涂片评估与全血细胞计数检查相结合对猛禽很重要。
