Hu Tingli, Chen Guotao, Xu Zhen, Luo Site, Wang Hui, Li Chunlin, Shan Lei, Zhang Baowei
School of Life Sciences, Anhui University, Hefei 230601, China.
School of Life Sciences, Xiamen University, Xiamen 361102, China.
Animals (Basel). 2022 Aug 8;12(15):2004. doi: 10.3390/ani12152004.
Yellow-throated bunting is a small migratory songbird unique to the Palearctic region. However, the genetic studies of this species remain limited, with no nuclear genomic sequence reported to date. In this study, the genomic DNA from the bird was sequenced in long reads using Nanopore sequencing technology. Combining short-read sequencing, the genome was well-assembled and annotated. The final length of the assembly is approximately 1.14 Gb, with a scaffold N50 of 28.94 Mb. About 15,868 protein-coding genes were predicted, and 16.62% of the genome was identified as having repetitive elements. Comparative genomic analysis showed numerous expanded gene families and positively selected genes significantly enriched in those KEGG pathways that are associated with migratory behavior adaptation and immune response. Here, this newly generated de novo genome of the yellow-throated bunting using long reads provide the research community with a valuable resource for further studies of population genetic diversity and genome evolution in this species.
黄喉鹀是古北界特有的一种小型候鸟。然而，对该物种的遗传学研究仍然有限，迄今为止尚无核基因组序列报道。在本研究中，利用纳米孔测序技术对该鸟类的基因组DNA进行了长读长测序。结合短读长测序，对基因组进行了良好的组装和注释。组装后的最终长度约为1.14 Gb，支架N50为28.94 Mb。预测了约15,868个蛋白质编码基因，基因组的16.62%被鉴定为具有重复元件。比较基因组分析显示，大量基因家族发生了扩张，正选择基因在与迁徙行为适应和免疫反应相关的KEGG通路中显著富集。在此，利用长读长新生成的黄喉鹀基因组为研究群体提供了宝贵资源，有助于进一步研究该物种的群体遗传多样性和基因组进化。
