LOEWE-Centre for Translational Biodiversity Genomics (LOEWE-TBG), Senckenberg Nature Research Society, Frankfurt am Main 60325, Germany.
Department of Zoology and Animal Cell Biology, University of the Basque Country (UPV-EHU), Vitoria-Gasteiz 01006, Spain.
G3 (Bethesda). 2021 Aug 7;11(8). doi: 10.1093/g3journal/jkab180.
Among all molluscs, land snails are a scientifically and economically interesting group comprising edible species, alien species and agricultural pests. Yet, despite their high diversity, the number of genome drafts publicly available is still scarce. Here, we present the draft genome assembly of the land snail Candidula unifasciata, a widely distributed species along central Europe, belonging to the Geomitridae family, a highly diversified taxon in the Western-Palearctic region. We performed whole genome sequencing, assembly and annotation of an adult specimen based on PacBio and Oxford Nanopore long read sequences as well as Illumina data. A genome draft of about 1.29 Gb was generated with a N50 length of 246 kb. More than 60% of the assembled genome was identified as repetitive elements. In total, 22,464 protein-coding genes were identified in the genome, of which 62.27% were functionally annotated. This is the first assembled and annotated genome for a geometrid snail and will serve as reference for further evolutionary, genomic and population genetic studies of this important and interesting group.
在所有软体动物中，陆地蜗牛是一个在科学和经济上都很有趣的群体，包括可食用的物种、外来物种和农业害虫。然而，尽管它们具有高度的多样性，但公开提供的基因组草案数量仍然很少。在这里，我们展示了分布广泛的欧洲中部陆地蜗牛 Candidula unifasciata 的基因组草图，它属于 Geomitridae 科，是西古北区高度多样化的分类群。我们基于 PacBio 和 Oxford Nanopore 长读序列以及 Illumina 数据对一个成年样本进行了全基因组测序、组装和注释。生成了一个约 12.9 亿碱基对的基因组草案，N50 长度为 246 千碱基对。组装基因组的 60%以上被鉴定为重复元件。总共鉴定出 22464 个编码蛋白的基因，其中 62.27%具有功能注释。这是第一个几何形蜗牛的组装和注释基因组，将作为进一步研究这个重要而有趣的群体的进化、基因组和群体遗传学的参考。
