来自大肠杆菌的精氨酸、甲酰甲硫氨酸-酪氨酸和甘氨酸转运RNA的结晶

Crystallization of arginine-, formylmethionine-tyrosine-, and glycine-transfer RNAs from Escherichia coli.

作者信息

Morikawa K, Sakamaki T, Nishimura Y, Mitsui Y, Aoki K, Iitaka Y, Tsuboi M, Nishimura S

出版信息

J Biochem. 1978 Aug;84(2):369-75. doi: 10.1093/oxfordjournals.jbchem.a132137.

DOI:10.1093/oxfordjournals.jbchem.a132137

Abstract

Crystals as large as 0.5 X 0.3 X 0.2 mm of purified arginine-transfer RNA from Escherichia coli have been prepared by a vapour diffusion method. X-ray diffraction photographs showed that the crystals gave reflections up to 3.7 A spacing. They have a trigonal space group P31 2 1 (OR P32 2 1) and cell-dimensions a=97.2, b=97.2, c=94.8A. Crystals of a mercury derivative of this transfer RNA have also been obtained, and an X-ray diffusion photography of one of them is presented. Formylmethionine-transfer RNA from E. coli was crystallized in various forms, and the appearance of the polymorphs was found to depend upon the amount of spermine in the solution from which the crystallization took place. Crystals of tyrosine-transfer RNA and glycine-transfer RNA have also been obtained.

摘要

通过气相扩散法制备出了来自大肠杆菌的纯化精氨酸转移核糖核酸晶体，其尺寸达0.5×0.3×0.2毫米。X射线衍射照片显示，这些晶体产生了间距达3.7埃的反射。它们属于三方晶系空间群P31 2 1（或P32 2 1），晶胞参数a = 97.2、b = 97.2、c = 94.8埃。还获得了这种转移核糖核酸汞衍生物的晶体，并展示了其中一个的X射线衍射照片。来自大肠杆菌的甲酰甲硫氨酸转移核糖核酸以多种形式结晶，发现多晶型物的外观取决于结晶所用溶液中精胺的含量。也已获得酪氨酸转移核糖核酸和甘氨酸转移核糖核酸的晶体。