Hampel A, Labanauskas M, Connors P G, Kirkegard L, RajBhandary U L, Sigler P B, Bock R M
Science. 1968 Dec 20;162(3860):1384-7. doi: 10.1126/science.162.3860.1384.
Reproducible conditions have been developed for crystallization of transfer RNA. The conditions may be applicable to many pure transfer RNA species since identical procedures (except for initial transfer-RNA concentration) yielded good crystals from both yeast and Escherichia coli transfer RNA. These crystals, which must be kept at temperatures below about 10 degrees C and handled in vapor of controlled alcohol concentration, have been studied by x-ray crystallography. The availability of crystals of a nucleic acid opens a route for extending knowledge of the tertiary structure of transfer RNA and its relation to important biological functions.
已经开发出用于转运RNA结晶的可重复条件。由于相同的程序（除了初始转运RNA浓度）能从酵母和大肠杆菌的转运RNA中都得到良好的晶体，所以这些条件可能适用于许多纯的转运RNA种类。这些晶体必须保存在约10摄氏度以下的温度，并在可控酒精浓度的蒸汽中处理，已经通过X射线晶体学对其进行了研究。核酸晶体的可得性为扩展对转运RNA三级结构及其与重要生物学功能关系的认识开辟了一条途径。
