Claro-Cala Carmen M, Grao-Cruces Elena, Toscano Rocio, Millan-Linares Maria C, Montserrat-de la Paz Sergio, Martin Maria E
Department of Pharmacology, Pediatric and Radiology, Faculty of Medicine, Universidad de Sevilla, Av. Sanchez Pizjuan s/n, 41009 Seville, Spain.
Department of Medical Biochemistry, Molecular Biology and Immunology, School of Medicine, Universidad de Sevilla, Av. Sanchez Pizjuan s/n, 41009 Seville, Spain.
Foods. 2022 Aug 7;11(15):2366. doi: 10.3390/foods11152366.
Seeds from non-drug varieties of hemp ( L.) have been used for traditional medicine, food, and fiber production. Our study shows that phytol obtained from hemp seed oil (HSO) exerts anti-inflammatory activity in human monocyte-macrophages. Fresh human monocytes and human macrophages derived from circulating monocytes were used to evaluate both plasticity and anti-inflammatory effects of phytol from HSO at 10-100 mM using FACS analysis, ELISA, and RT-qPCR methods. The quantitative study of the acyclic alcohol fraction isolated from HSO shows that phytol is the most abundant component (167.59 ± 1.81 mg/Kg of HSO). Phytol was able to skew monocyte-macrophage plasticity toward the anti-inflammatory non-classical CD14CD16 monocyte phenotype and toward macrophage M2 (CD200R and MRC-1), as well as to reduce the production of IL-1β, IL-6, and TNF-α, diminishing the inflammatory competence of mature human macrophages after lipopolysaccharide (LPS) treatment. These findings point out for the first time the reprogramming and anti-inflammatory activity of phytol in human monocyte-macrophages. In addition, our study may help to understand the mechanisms by which phytol from HSO contributes to the constant and progressive plasticity of the human monocyte-macrophage linage.
来自非药用大麻品种（大麻属）的种子已被用于传统医学、食品和纤维生产。我们的研究表明，从大麻籽油（HSO）中提取的叶绿醇在人单核细胞-巨噬细胞中具有抗炎活性。使用FACS分析、ELISA和RT-qPCR方法，以新鲜的人单核细胞和源自循环单核细胞的人巨噬细胞为研究对象，评估10-100 mM浓度下HSO中叶绿醇的可塑性和抗炎作用。对从HSO中分离出的无环醇馏分进行的定量研究表明，叶绿醇是最丰富的成分（167.59±1.81 mg/Kg的HSO）。叶绿醇能够使单核细胞-巨噬细胞的可塑性偏向抗炎的非经典CD14CD16单核细胞表型和巨噬细胞M2（CD200R和MRC-1），还能减少脂多糖（LPS）处理后成熟人巨噬细胞中IL-1β、IL-6和TNF-α的产生，降低其炎症能力。这些发现首次指出了叶绿醇在人单核细胞-巨噬细胞中的重编程和抗炎活性。此外，我们的研究可能有助于理解HSO中的叶绿醇对人单核细胞-巨噬细胞谱系持续和渐进可塑性的作用机制。
