大麻亚种大麻（大麻）提取物和大麻二酚的抗炎和牙龈伤口愈合活性：一项体外研究。

Anti-inflammation and gingival wound healing activities of Cannabis sativa L. subsp. sativa (hemp) extract and cannabidiol: An in vitro study.

机构信息

Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, Thailand.

Faculty of Public Health and Allied Health Sciences, Praboromarajchanok Institute, Ministry of Public Health, Phitsanulok 65130, Thailand.

出版信息

Arch Oral Biol. 2022 Aug;140:105464. doi: 10.1016/j.archoralbio.2022.105464. Epub 2022 May 19.

DOI:10.1016/j.archoralbio.2022.105464

PMID:35623115

Abstract

OBJECTIVE

To evaluate the anti-inflammatory and gingival wound healing activities of Cannabis sativa L. subsp. sativa (hemp) extract and cannabidiol (CBD).

DESIGN

The cellular bioactivities of hemp extract and CBD were determined the inhibition of TNF-α and IL-1β in LPS-induced murine macrophage (RAW 264.7) cells by using ELISA while wound healing activity in human gingival fibroblast (HGF-1) cells was performed by a scratch test assay. The cytotoxicity was also concerned and evaluated by MTT assay.

RESULTS

The hemp extract and CBD significantly decreased TNF-α release by up to 91.05 ± 2.91% and 50.78 ± 7.21% of LPS activity, respectively, in a dose-dependent manner, compared to 10 µg/mL hydrocortisone (61.67 ± 3.79%). The hemp extract and CBD also significantly decreased IL-1β release, also in dose-dependent response, up to 78.03 ± 3.34% and 85.87 ± 1.11% of LPS activity, respectively, compared to 5 µg/mL hydrocortisone (80.81 ± 3.55%). The mean percentage of closure of the wound area was 27.92 ± 1.21% when exposed to 5 µg/mL hemp extract and 33.49 ± 1.67% when exposed to 0.5 µg/mL CBD, compared to 24.34 ± 2.29% for non-treated control.

CONCLUSIONS

Our study demonstrates that both hemp extract and CBD can inhibit TNF-α and IL-1β production in LPS-induced RAW 264.7 cells and promote wound healing in HGF-1 cells. This is the first to show that short-term exposure to hemp extract and CBD promoted gingival fibroblast wound healing, demonstrating that hemp extract and CBD have potential benefits in the treatment of oral inflammation and ulcers.

摘要

目的

评估大麻亚种（大麻）提取物和大麻二酚（CBD）的抗炎和牙龈伤口愈合活性。

设计

通过 ELISA 测定大麻提取物和 CBD 对脂多糖（LPS）诱导的小鼠巨噬细胞（RAW 264.7）细胞中 TNF-α 和 IL-1β 的抑制作用，用人牙龈成纤维细胞（HGF-1）细胞划痕试验测定伤口愈合活性。还通过 MTT 测定法关注和评估细胞毒性。

结果

与 10μg/mL 氢化可的松（61.67±3.79%）相比，大麻提取物和 CBD 呈剂量依赖性地分别将 TNF-α 的释放降低多达 91.05±2.91%和 50.78±7.21%，抑制 LPS 活性。大麻提取物和 CBD 还显著降低了 IL-1β 的释放，也呈剂量依赖性反应，分别高达 LPS 活性的 78.03±3.34%和 85.87±1.11%，与 5μg/mL 氢化可的松（80.81±3.55%）相比。当暴露于 5μg/mL 大麻提取物时，伤口区域的闭合百分比平均值为 27.92±1.21%，当暴露于 0.5μg/mL CBD 时，闭合百分比平均值为 33.49±1.67%，而非处理对照的闭合百分比平均值为 24.34±2.29%。

结论

我们的研究表明，大麻提取物和 CBD 均可抑制 LPS 诱导的 RAW 264.7 细胞中 TNF-α 和 IL-1β 的产生，并促进 HGF-1 细胞中的伤口愈合。这是首次表明短期暴露于大麻提取物和 CBD 可促进牙龈成纤维细胞伤口愈合，表明大麻提取物和 CBD 在治疗口腔炎症和溃疡方面具有潜在益处。