Department of Water Ecology, Faculty of Biology, University of Białystok, Ciołkowskiego 1J, 15-245 Bialystok, Poland.
Int J Environ Res Public Health. 2022 Jul 31;19(15):9408. doi: 10.3390/ijerph19159408.
The aim of this study was to determine the effect of humic acid on the growth and metabolism of , a common waterborne pathogenic yeast. At 10-20 mg/L, humic acid caused the greatest increase in biomass and compactness of proteins and monosaccharides, both in cells and in extracellular secretion of the yeast. At higher humic acid concentrations (40-80 mg/L), cells still had higher protein levels compared to control, but showed reduced levels of metabolites and inhibited growth, and a significant increase in the activity of antioxidant enzymes, indicating a toxic effect of the humic acid. The increase in protein content in the cells of C. albicans combined with an increase in the activity of antioxidant enzymes may indicate that the studied yeast excels in conditions of high water enrichment with low availability of organic matter. This indicates that is capable of breaking down organic matter that other microorganisms cannot cope with, and for this reason, this yeast uses carbon sources that are not available to other microorganisms. This indicates that this fungus plays an important role in the organic carbon sphere to higher trophic levels, and is common in water polluted with organic matter.
本研究旨在确定腐殖酸对一种常见水生病原性酵母的生长和代谢的影响。在 10-20mg/L 时，腐殖酸引起细胞内和细胞外分泌的生物量和蛋白质及单糖的紧密度增加最大。在较高的腐殖酸浓度（40-80mg/L）下，与对照相比，细胞内仍具有较高的蛋白质水平，但代谢物水平降低，生长受到抑制，抗氧化酶的活性显著增加，表明腐殖酸具有毒性作用。白色念珠菌细胞内蛋白质含量的增加以及抗氧化酶活性的增加可能表明，研究中的酵母在高水分富集而有机物可用性低的条件下表现出色。这表明该酵母能够分解其他微生物无法处理的有机物，因此，该酵母利用其他微生物无法利用的碳源。这表明该真菌在有机碳向更高营养级的转化中发挥着重要作用，并且在有机物污染的水中很常见。
