Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education, Agricultural College, Yangzhou University, Yangzhou 225009, China.
Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Agricultural College, Yangzhou University, Yangzhou 225009, China.
Int J Mol Sci. 2022 Jul 27;23(15):8296. doi: 10.3390/ijms23158296.
Grain weight is a key trait that determines rice quality and yield, and it is primarily controlled by quantitative trait loci (QTL). Recently, attention has been paid to minor QTLs. A minor effect QTL that controls grain weight was previously identified in a set of chromosomal fragment substitution lines (CSSLs) derived from Nipponbare (NPB)/93-11. Compared to NPB, the single segment substitution line (SSSL) N83 carrying the introgression exhibited an increase in grain length and width and a 4.5% increase in grain weight. Meanwhile, N83 was backcrossed to NPB to create a separating population, , a QTL distinct from , which was detected between markers G31 and G32. Twelve near-isogenic lines (NILs) from the BCF population and progeny of five NILs from the BCF population were genotyped and phenotyped, resulting in the fine mapping of the minor effect QTL to the approximately 86.2-kb region between markers G72 and G32. Further sequence comparisons and expression analysis confirmed that five genes, including , , , , and , were considered as the candidate genes underlying . These results provide a crucial foundation for further cloning of and molecular breeding design in rice.
粒重是决定水稻品质和产量的关键性状，主要受数量性状位点（QTL）控制。最近，人们对微效 QTL 越来越关注。先前在一套源于 Nipponbare（NPB）/93-11 的染色体片段代换系（CSSLs）中鉴定到一个控制粒重的微效 QTL。与 NPB 相比，携带导入片段的单片段代换系（SSSL）N83 的粒长和粒宽增加，粒重增加 4.5%。同时，N83 回交至 NPB 构建分离群体 ，该群体中检测到一个不同于 的 QTL，位于标记 G31 和 G32 之间。来自 BCF 群体的 12 个近等基因系（NILs）及其来自 BCF 群体的 5 个 NILs 的后代进行了基因型和表型分析，将微效 QTL 精细定位到标记 G72 和 G32 之间约 86.2-kb 的区域。进一步的序列比较和表达分析证实，包括 、 、 、 和 在内的五个基因被认为是 的候选基因。这些结果为进一步克隆 和在水稻中的分子育种设计提供了重要基础。
