大豆种子含油量重要数量性状位点的鉴定。
Identification of an important QTL for seed oil content in soybean.
作者信息
Li Bing, Peng Jingyu, Wu Yueying, Hu Quan, Huang Wenxuan, Yuan Zhihui, Tang Xiaofei, Cao Dan, Xue Yongguo, Luan Xiaoyan, Hou Jingjing, Liu Xinlei, Sun Lianjun
机构信息
College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193 China.
Sanya Institute of China Agricultural University, Sanya, 572000 China.
出版信息
Mol Breed. 2023 May 19;43(6):43. doi: 10.1007/s11032-023-01384-2. eCollection 2023 Jun.
UNLABELLED
Seed oil content is one of the most important quantitative traits in soybean () breeding. Here, we constructed a high-density single nucleotide polymorphism linkage map using two genetically similar parents, Heinong 84 and Kenfeng 17, that differ dramatically in their seed oil contents, and performed quantitative trait loci (QTL) mapping of seed oil content in a recombinant inbred line (RIL) population derived from their cross. We detected five QTL related to seed oil content distributed on five chromosomes. The QTL for seed oil content explained over 10% of the phenotypic variation over two years. This QTL was mapped to an interval containing 20 candidate genes, including a previously reported gene, soybean () encoding an E3 ubiquitin ligase. Notably, two short sequences were inserted in the coding region of KF 17 compared to that of HN 84, resulting in a longer protein variant in KF 17. Our results thus provide information for uncovering the genetic mechanisms determining seed oil content in soybean, as well as identifying an additional QTL and highlighting as candidate gene for modulating seed oil content in soybean.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s11032-023-01384-2.
未标注
种子油含量是大豆育种中最重要的数量性状之一。在此，我们利用两个遗传相似但种子油含量差异显著的亲本黑农84和垦丰17构建了高密度单核苷酸多态性连锁图谱，并对其杂交衍生的重组自交系（RIL）群体的种子油含量进行了数量性状位点（QTL）定位。我们检测到5个与种子油含量相关的QTL，分布在5条染色体上。种子油含量的QTL在两年内解释了超过10%的表型变异。该QTL被定位到一个包含20个候选基因的区间，其中包括一个先前报道的基因，大豆编码一种E3泛素连接酶。值得注意的是，与HN 84相比，KF 17的编码区插入了两个短序列，导致KF 17中出现了一个更长的蛋白质变体。因此，我们的结果为揭示大豆种子油含量的遗传机制提供了信息，同时鉴定了一个额外的QTL，并突出了作为调节大豆种子油含量的候选基因。
补充信息
在线版本包含可在10.1007/s11032-023-01384-2获取的补充材料。
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