Institute of Metabolic and Cardiovascular Disease, Inserm UMR1297, University of Toulouse 3, 31024 Toulouse, France.
Platelet Proteomics Group, Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), Universidade de Santiago de Compostela and Instituto de Investigación Sanitaria de Santiago (IDIS), 15706 Santiago de Compostela, Spain.
Int J Mol Sci. 2022 Jul 28;23(15):8326. doi: 10.3390/ijms23158326.
Obesity is associated with a pro-inflammatory and pro-thrombotic state that supports atherosclerosis progression and platelet hyper-reactivity. During the last decade, the platelet lipidome has been considered a treasure trove, as it is a source of biomarkers for preventing and treating different pathologies. The goal of the present study was to determine the lipid profile of platelets from non-diabetic, severely obese patients compared with their age- and sex-matched lean controls. Lipids from washed platelets were isolated and major phospholipids, sphingolipids and neutral lipids were analyzed either by gas chromatography or by liquid chromatography coupled to mass spectrometry. Despite a significant increase in obese patient's plasma triglycerides, there were no significant differences in the levels of triglycerides in platelets among the two groups. In contrast, total platelet cholesterol was significantly decreased in the obese group. The profiling of phospholipids showed that phosphatidylcholine and phosphatidylethanolamine contents were significantly reduced in platelets from obese patients. On the other hand, no significant differences were found in the sphingomyelin and ceramide levels, although there was also a tendency for reduced levels in the obese group. The outline of the glycerophospholipid and sphingolipid molecular species (fatty-acyl profiles) was similar in the two groups. In summary, these lipidomics data indicate that platelets from obese patients have a unique lipid fingerprint that may guide further studies and provide mechanistic-driven perspectives related to the hyperactivate state of platelets in obesity.
肥胖与促炎和促血栓状态相关，这种状态支持动脉粥样硬化的进展和血小板的高反应性。在过去的十年中，血小板脂质组学被认为是一个宝库，因为它是预防和治疗不同病理的生物标志物的来源。本研究的目的是确定与年龄和性别相匹配的瘦对照组相比，非糖尿病、严重肥胖患者血小板的脂质谱。分离了经洗涤的血小板中的脂质，并通过气相色谱或液相色谱-质谱联用分析了主要的磷脂、鞘脂和中性脂质。尽管肥胖患者的血浆甘油三酯水平显著增加，但两组血小板中的甘油三酯水平没有显著差异。相比之下，肥胖组的总血小板胆固醇显著降低。磷脂谱分析表明，肥胖患者血小板中的磷脂酰胆碱和磷脂酰乙醇胺含量显著降低。另一方面，鞘磷脂和神经酰胺水平没有显著差异，尽管肥胖组也有降低的趋势。甘油磷脂和鞘脂的分子种类（脂肪酸图谱）轮廓在两组中相似。总之，这些脂质组学数据表明，肥胖患者的血小板具有独特的脂质特征，可能为进一步的研究提供指导，并提供与肥胖症中血小板过度激活状态相关的机制驱动的观点。
