GTP 结合蛋白 Gtr1 与 ASF1 合作调控. 的无性发育

GTP Binding Protein Gtr1 Cooperating with ASF1 Regulates Asexual Development in .

机构信息

Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian 271018, China.

College of Life Sciences, Shandong Normal University, Jinan 250014, China.

出版信息

Int J Mol Sci. 2022 Jul 28;23(15):8355. doi: 10.3390/ijms23158355.

DOI:10.3390/ijms23158355

PMID:35955500

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9369126/

Abstract

The Gtr1 protein was a member of the RagA subfamily of the Ras-like small GTPase superfamily and involved in phosphate acquisition, ribosome biogenesis and epigenetic control of gene expression in yeast. However, Gtr1 regulation sexual or asexual development in filamentous fungi is barely accepted. In the study, SeGtr1, identified from , could manipulate mycelial growth, nuclear distribution of mycelium and the morphology of conidia in silenced strains compared with its overexpression transformants, while the sexual activity of silenced strains were unchanged. SeASF1, a H3/H4 chaperone, participated in nucleosome assembly/disassembly, DNA replication and transcriptional regulation. Our experiments showed that deletion mutants produced the hyphal fusion and abnormal conidia. Notably, we characterized that was down-regulated in Se∆ mutants and was also down-regulated in Si strains. We further confirmed that SeGtr1 interacted with SeASF1 or SeH4 in vivo and vitro, respectively. Thus, SeGtr1 can cooperate with SeASF1 to modulate asexual development in .

摘要

Gtr1 蛋白是 Ras 样小分子 GTP 酶超家族 RagA 亚家族的成员，参与酵母中磷酸盐的获取、核糖体的生物发生以及基因表达的表观遗传调控。然而，Gtr1 在丝状真菌中的性或无性发育的调控作用尚未被广泛接受。在本研究中，从中鉴定出的 SeGtr1 可以在沉默菌株中操纵菌丝生长、菌丝的核分布和分生孢子的形态，与过表达转化体相比，而沉默菌株的有性活性没有变化。SeASF1 是一种 H3/H4 伴侣蛋白，参与核小体组装/拆卸、DNA 复制和转录调控。我们的实验表明，缺失突变体产生菌丝融合和异常分生孢子。值得注意的是，我们发现在 Se∆ 突变体中下调，而在 Si 菌株中也下调。我们进一步证实 SeGtr1 可以分别在体内和体外与 SeASF1 或 SeH4 相互作用。因此，SeGtr1 可以与 SeASF1 合作调节中的无性发育。