Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China.
Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China.
Int J Mol Sci. 2022 Jul 28;23(15):8369. doi: 10.3390/ijms23158369.
C-type lectins (CTLs) are widely distributed in mammals, insects, and plants, which act as pattern recognition receptors (PRRs) to recognize pathogens and initiate immune responses. In this study, we identified a C-type lectin gene called from the silkworm . Its open reading frame (ORF) encodes 314 amino acids, which contain dual tandem C-type lectin-like domain (CTLD). is highly expressed in the fat body and is significantly induced at 24 h after BmNPV infection. Moreover, overexpression of dramatically inhibited the proliferation of BmNPV, and knockdown assay via siRNA further validated the inhibition of on viral proliferation. In addition, transcript level detection of apoptosis-related genes and observation of apoptosis bodies implied that overexpression of promoted BmNPV-induced apoptosis. Immunofluorescence analysis indicated that BmIML-2 distributed throughout the cytoplasm and was slightly concentrated in the cell membrane. Taken together, our results suggest that BmIML-2 could inhibit in the proliferation of BmNPV by facilitating cell apoptosis.
C 型凝集素 (CTLs) 广泛分布于哺乳动物、昆虫和植物中，作为模式识别受体 (PRRs) 识别病原体并启动免疫反应。本研究从家蚕中鉴定出一个 C 型凝集素基因，命名为 。其开放阅读框 (ORF) 编码 314 个氨基酸，包含双串联 C 型凝集素样结构域 (CTLD)。 在脂肪体中高度表达，并在 BmNPV 感染后 24 小时显著诱导。此外， 的过表达显著抑制了 BmNPV 的增殖，通过 siRNA 的敲低实验进一步验证了 对病毒增殖的抑制作用。此外，通过检测凋亡相关基因的转录水平和观察凋亡小体，表明 过表达促进了 BmNPV 诱导的凋亡。免疫荧光分析表明 BmIML-2 分布于整个细胞质中，在细胞膜上略有集中。综上所述，我们的研究结果表明，BmIML-2 通过促进细胞凋亡来抑制 BmNPV 的增殖。
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