Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China.
Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, The Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212100, China.
Int J Mol Sci. 2023 Dec 28;25(1):402. doi: 10.3390/ijms25010402.
Trehalose is a non-reducing disaccharide and participates in physiological activities such as organ formation, energy metabolism, and stress resistance in insects. The 1 gene family is mainly involved in in the sugar metabolism of silkworm. In the present study, phylogenetic analysis divided 21 1 orthologs into three clades. These genes are equally distributed on the nine chromosomes. The cis-elements in the promoter regions of 1s indicated the possible function of 1s in response to hormones and environmental stimulus. The qPCR analysis showed the significantly different expression levels of 1s in different tissues and organs, indicating possible functional divergence. In addition, most 1s showed disturbed expression levels in response to silkworm nuclear polyhedrosis virus (BmNPV) stresses. Our results provide a clue for further functional dissection of the 1s in and implicate them as potential regulators of antiviral responses.
海藻糖是一种非还原双糖，参与昆虫的器官形成、能量代谢和抗逆性等生理活动。1 基因家族主要参与家蚕的糖代谢。本研究通过系统发育分析将 21 个 1 直系同源基因分为三个分支。这些基因均匀分布在 9 条染色体上。1s 启动子区域中的顺式元件表明 1s 可能参与了对激素和环境刺激的响应。qPCR 分析显示 1s 在不同组织和器官中的表达水平存在显著差异，表明可能存在功能分化。此外，大多数 1s 在响应家蚕核型多角体病毒（BmNPV）胁迫时表现出表达水平紊乱。我们的研究结果为进一步研究 1s 在 中的功能提供了线索，并暗示它们可能是抗病毒反应的潜在调控因子。
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