Department of Biology and Biological Engineering, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden.
Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden.
Int J Mol Sci. 2022 Jul 31;23(15):8517. doi: 10.3390/ijms23158517.
Given the strong potential of to produce lipids for use as renewable fuels and oleochemicals, it is important to gain in-depth understanding of the molecular mechanism underlying its lipid accumulation. As cellular growth rate affects biomass lipid content, we performed a comparative proteomic analysis of grown in nitrogen-limited chemostat cultures at different dilution rates. After confirming the correlation between growth rate and lipid accumulation, we were able to identify various cellular functions and biological mechanisms involved in oleaginousness. Inspection of significantly up- and downregulated proteins revealed nonintuitive processes associated with lipid accumulation in this yeast. This included proteins related to endoplasmic reticulum (ER) stress, ER-plasma membrane tether proteins, and arginase. Genetic engineering of selected targets validated that some genes indeed affected lipid accumulation. They were able to increase lipid content and were complementary to other genetic engineering strategies to optimize lipid yield.
鉴于 具有生产可再生燃料和油脂化学品所需脂质的巨大潜力，深入了解其脂质积累的分子机制非常重要。由于细胞生长速率会影响生物质脂质含量，因此我们对在不同稀释率下生长于氮限制恒化器培养物中的 进行了比较蛋白质组学分析。在确认生长速率与脂质积累之间的相关性后，我们能够鉴定出参与油脂生成的各种细胞功能和生物学机制。对显著上调和下调蛋白的检查揭示了与该酵母中脂质积累相关的非直观过程。其中包括与内质网（ER）应激、ER-质膜连接蛋白和精氨酸酶相关的蛋白质。对选定靶标的遗传工程验证了某些基因确实会影响脂质积累。它们能够增加脂质含量，并与其他遗传工程策略互补，以优化脂质产量。
