Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.
Biotechnol Bioeng. 2021 Oct;118(10):3640-3654. doi: 10.1002/bit.27859. Epub 2021 Jun 21.
Concerns about climate change and the search for renewable energy sources together with the goal of attaining sustainable product manufacturing have boosted the use of microbial platforms to produce fuels and high-value chemicals. In this regard, Yarrowia lipolytica has been known as a promising yeast with potentials in diverse array of biotechnological applications such as being a host for different oleochemicals, organic acid, and recombinant protein production. Having a rapidly increasing number of molecular and genetic tools available, Y. lipolytica has been well studied amongst oleaginous yeasts and metabolic engineering has been used to explore its potentials. More recently, with the advancement in systems biotechnology and the implementation of mathematical modeling and high throughput omics data-driven approaches, in-depth understanding of cellular mechanisms of cell factories have been made possible resulting in enhanced rational strain design. In case of Y. lipolytica, these systems-level studies and the related cutting-edge technologies have recently been initiated which is expected to result in enabling the biotechnology sector to rationally engineer Y. lipolytica-based cell factories with favorable production metrics. In this regard, here, we highlight the current status of systems metabolic engineering research and assess the potential of this yeast for future cell factory design development.
对气候变化的担忧和对可再生能源的寻找，以及实现可持续产品制造的目标，都推动了微生物平台在生产燃料和高价值化学品方面的应用。在这方面，解脂耶氏酵母已被公认为一种很有前途的酵母，在各种生物技术应用中具有潜力，如作为不同油脂化学品、有机酸和重组蛋白生产的宿主。随着越来越多的分子和遗传工具的出现，解脂耶氏酵母得到了充分的研究，代谢工程也被用于探索其潜力。最近，随着系统生物技术的进步，以及数学建模和高通量组学数据驱动方法的实施，细胞工厂的细胞机制得到了深入的了解，从而实现了理性的菌株设计的增强。就解脂耶氏酵母而言，这些系统水平的研究和相关的前沿技术最近已经开始，有望使生物技术部门能够合理地设计基于解脂耶氏酵母的细胞工厂，以获得有利的生产指标。在这方面，我们在这里强调了系统代谢工程研究的现状，并评估了这种酵母在未来细胞工厂设计开发中的潜力。
