College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
Department of Bioengineering and Imperial College Centre for Synthetic Biology, Imperial College London, London SW7 2AZ, UK.
Bioresour Technol. 2020 Oct;313:123707. doi: 10.1016/j.biortech.2020.123707. Epub 2020 Jun 21.
Current energy security and climate change policies encourage the development and utilization of bioenergy. Oleaginous yeasts provide a particularly attractive platform for the sustainable production of biofuels and industrial chemicals due to their ability to accumulate high amounts of lipids. In particular, microbial lipids in the form of triacylglycerides (TAGs) produced from renewable feedstocks have attracted considerable attention because they can be directly used in the production of biodiesel and oleochemicals analogous to petrochemicals. As an oleaginous yeast that is generally regarded as safe, Yarrowia lipolytica has been extensively studied, with large amounts of data on its lipid metabolism, genetic tools, and genome sequencing and annotation. In this review, we highlight the newest strategies for increasing lipid accumulation using metabolic engineering and summarize the research advances on the overaccumulation of lipids in Y. lipolytica. Finally, perspectives for future engineering approaches are proposed.
当前的能源安全和气候变化政策鼓励生物能源的开发和利用。由于能够积累大量的油脂，产油酵母为生物燃料和工业化学品的可持续生产提供了一个特别有吸引力的平台。特别是，来自可再生原料的微生物油脂，以三酰基甘油（TAG）的形式，已经引起了相当大的关注，因为它们可以直接用于生产类似于石油化工产品的生物柴油和油脂化学品。解脂耶氏酵母作为一种被普遍认为安全的产油酵母，已经得到了广泛的研究，其脂质代谢、遗传工具、基因组测序和注释方面都有大量的数据。在这篇综述中，我们强调了使用代谢工程来提高油脂积累的最新策略，并总结了在解脂耶氏酵母中过度积累油脂的研究进展。最后，提出了未来工程方法的展望。
