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高、低胚性巴西橡胶树基因型的比较转录组分析揭示了植物激素在体细胞胚胎发生中的作用。

Comparative transcriptome analysis of high- and low-embryogenic Hevea brasiliensis genotypes reveals involvement of phytohormones in somatic embryogenesis.

机构信息

The Center of Rubber Research, Yunnan Institute of Tropical Crops, Xishuangbanna, China.

出版信息

BMC Plant Biol. 2023 Oct 13;23(1):489. doi: 10.1186/s12870-023-04432-3.

DOI:10.1186/s12870-023-04432-3
PMID:37828441
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10571474/
Abstract

BACKGROUND

Rubber plant (Hevea brasiliensis) is one of the major sources of latex. Somatic embryogenesis (SE) is a promising alterative to its propagation by grafting and seed. Phytohormones have been shown to influence SE in different plant species. However, limited knowledge is available on the role of phytohormones in SE in Hevea. The anther cultures of two Hevea genotypes (Yunyan 73477-YT and Reken 628-RT) with contrasting SE rate were established and four stages i.e., anthers (h), anther induced callus (y), callus differentiation state (f), and somatic embryos (p) were studied. UPLC-ESI-MS/MS and transcriptome analyses were used to study phytohormone accumulation and related expression changes in biosynthesis and signaling genes.

RESULTS

YT showed higher callus induction rate than RT. Of the two genotypes, only YT exhibited successful SE. Auxins, cytokinins (CKs), abscisic acid (ABA), jasmonic acid (JA), salicylic acid (SA), gibberellins (GAs), and ethylene (ETH) were detected in the two genotypes. Indole-3-acetic acid (IAA), CKs, ABA, and ETH had notable differences in the studied stages of the two genotypes. The differentially expressed genes identified in treatment comparisons were majorly enriched in MAPK and phytohormone signaling, biosynthesis of secondary metabolites, and metabolic pathways. The expression changes in IAA, CK, ABA, and ETH biosynthesis and signaling genes confirmed the differential accumulation of respective phytohormones in the two genotypes.

CONCLUSION

These results suggest potential roles of phytohormones in SE in Hevea.

摘要

背景

橡胶树（Hevea brasiliensis）是天然橡胶的主要来源之一。体细胞胚胎发生（SE）是一种有前途的替代嫁接和种子的繁殖方法。植物激素已被证明可以影响不同植物物种的 SE。然而，关于植物激素在橡胶树 SE 中的作用的知识有限。建立了两个具有不同 SE 率的橡胶树基因型（云燕 73477-YT 和瑞垦 628-RT）的花药培养，并研究了四个阶段，即花药（h）、花药诱导愈伤组织（y）、愈伤组织分化状态（f）和体细胞胚（p）。使用 UPLC-ESI-MS/MS 和转录组分析研究了生物合成和信号转导基因中植物激素的积累和相关表达变化。

结果

YT 的愈伤组织诱导率高于 RT。在这两个基因型中，只有 YT 表现出成功的 SE。在这两个基因型中检测到了生长素、细胞分裂素（CKs）、脱落酸（ABA）、茉莉酸（JA）、水杨酸（SA）、赤霉素（GA）和乙烯（ETH）。吲哚-3-乙酸（IAA）、CKs、ABA 和 ETH 在两个基因型的研究阶段有显著差异。处理比较中鉴定的差异表达基因主要富集在 MAPK 和植物激素信号转导、次生代谢物生物合成和代谢途径中。IAA、CK、ABA 和 ETH 生物合成和信号转导基因的表达变化证实了两个基因型中各自植物激素的差异积累。

结论

这些结果表明植物激素在橡胶树 SE 中可能发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/970f/10571474/c1a45ab1518f/12870_2023_4432_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/970f/10571474/6a09228d5251/12870_2023_4432_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/970f/10571474/7630f172175c/12870_2023_4432_Fig5_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/970f/10571474/dc150fa20008/12870_2023_4432_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/970f/10571474/21b79f9a152c/12870_2023_4432_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/970f/10571474/cb4f25f12526/12870_2023_4432_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/970f/10571474/c1a45ab1518f/12870_2023_4432_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/970f/10571474/6a09228d5251/12870_2023_4432_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/970f/10571474/a44e55c3ac9d/12870_2023_4432_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/970f/10571474/8230cb0dc4b8/12870_2023_4432_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/970f/10571474/61104f3c59f0/12870_2023_4432_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/970f/10571474/7630f172175c/12870_2023_4432_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/970f/10571474/4af981c928a3/12870_2023_4432_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/970f/10571474/dc150fa20008/12870_2023_4432_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/970f/10571474/21b79f9a152c/12870_2023_4432_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/970f/10571474/cb4f25f12526/12870_2023_4432_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/970f/10571474/c1a45ab1518f/12870_2023_4432_Fig10_HTML.jpg

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