Department of Forest Biological Sciences, College of Forestry and Natural Resources, University of the Philippines Los Baños, Laguna 4031, Philippines.
Department of Environment and Forest Resources, College of Agriculture and Life Science, Chungnam National University, Daejeon 34134, Korea.
Int J Mol Sci. 2022 Aug 3;23(15):8640. doi: 10.3390/ijms23158640.
In this study, we investigated the effects of water-deficit stress on the leaf anatomical traits, physiological traits, and stem starch content in Carruth and Murray by subjecting their seedlings to well-watered (WW) and water-deficit stress (WS) treatments. The water stress-induced changes in trichome density, trichome-to-stomata ratio, mesophyll thickness, vein density, vein distance, vein loopiness, vessel diameter, transpiration (E), stomatal conductance (g), water use efficiency (WUE), and starch content were analyzed between two time points. While trichome density did not vary between treatments in , it dramatically increased in (62.63-98.96 trichomes mm) at the final week. The WS-treated seedlings had a thicker palisade mesophyll (162.85-169.56 µm) than the WW-treated samples (118.56-132.25 µm) in both species. The vein density and loopiness increased significantly in the WS-treated seedlings. Small-sized vessels (10-50 µm) were more frequent in the WS than the WW in . The E, g, WUE, and starch content declined significantly in the WS-treated seedlings compared with WW-treated samples in both species. Further, principal component analysis revealed significant relationships between anatomical and physiological traits, particularly in the WS-treated seedlings of . The coordinated changes in leaf anatomical traits, physiological traits, and stem starch content indicate an important role in the survival of and seedlings in water-deficit stress environments, although may show higher survivability under prolonged water stress than .
在这项研究中，我们通过对 Carruth 和 Murray 的幼苗进行充分浇水（WW）和缺水胁迫（WS）处理，研究了水分胁迫对叶片解剖结构特征、生理特征和茎淀粉含量的影响。分析了两个时间点之间水胁迫诱导的绒毛密度、绒毛对气孔比、叶肉厚度、叶脉密度、叶脉间距、叶脉环度、导管直径、蒸腾（E）、气孔导度（g）、水分利用效率（WUE）和淀粉含量的变化。虽然 Carruth 的绒毛密度在处理之间没有差异，但在最后一周，它显著增加（62.63-98.96 个/毫米）。与 WW 处理的样本相比（118.56-132.25 微米），WS 处理的幼苗具有更厚的栅栏叶肉（162.85-169.56 微米）在两个物种中。叶脉密度和环度在 WS 处理的 Murray 幼苗中显著增加。在 WS 中，10-50 微米的小尺寸导管比 WW 更频繁。与 WW 处理的样本相比，E、g、WUE 和淀粉含量在 WS 处理的幼苗中显著下降，在两个物种中均如此。此外，主成分分析显示解剖结构和生理特征之间存在显著关系，特别是在 WS 处理的 Murray 幼苗中。叶片解剖结构特征、生理特征和茎淀粉含量的协调变化表明，在缺水胁迫环境中，Carruth 和 Murray 幼苗的生存具有重要作用，尽管在长期缺水胁迫下，Carruth 可能比 Murray 具有更高的存活率。
