Laboratoire Léon Brillouin LLB, CEA, CNRS UMR12, Université Paris Saclay, CEA Saclay, 91191 Gif-sur-Yvette, France.
Department of Molecular Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland.
Int J Mol Sci. 2022 Aug 5;23(15):8739. doi: 10.3390/ijms23158739.
Hfq is a pleiotropic regulator that mediates several aspects of bacterial RNA metabolism. The protein notably regulates translation efficiency and RNA decay in Gram-negative bacteria, usually via its interaction with small regulatory RNAs. Previously, we showed that the Hfq C-terminal region forms an amyloid-like structure and that these fibrils interact with membranes. The immediate consequence of this interaction is a disruption of the membrane, but the effect on Hfq structure was unknown. To investigate details of the mechanism of interaction, the present work uses different in vitro biophysical approaches. We show that the Hfq C-terminal region influences membrane integrity and, conversely, that the membrane specifically affects the amyloid assembly. The reported effect of this bacterial master regulator on membrane integrity is discussed in light of the possible consequence on small regulatory RNA-based regulation.
Hfq 是一种多效调节因子，介导细菌 RNA 代谢的几个方面。该蛋白特别通过与小调控 RNA 的相互作用来调节革兰氏阴性细菌的翻译效率和 RNA 降解。以前，我们表明 Hfq C 端区域形成类似淀粉样的结构，并且这些纤维与膜相互作用。这种相互作用的直接结果是破坏膜，但对 Hfq 结构的影响尚不清楚。为了研究相互作用的机制细节，本工作使用了不同的体外生物物理方法。我们表明，Hfq C 端区域影响膜的完整性，相反，膜特异性地影响淀粉样组装。根据对基于小调控 RNA 的调节的可能后果，讨论了这种细菌主要调节剂对膜完整性的影响。
Zotero 插件
