基因上游lncRNA作为子痫前期病因的特征分析

Characterization of the lncRNA Located Upstream of the Gene as an Etiology for Pre-Eclampsia.

作者信息

Yoshizawa Hikari, Nishizawa Haruki, Inagaki Hidehito, Hitachi Keisuke, Ohwaki Akiko, Sakabe Yoshiko, Ito Mayuko, Tsuchida Kunihiro, Sekiya Takao, Fujii Takuma, Kurahashi Hiroki

机构信息

Department of Obstetrics and Gynecology, School of Medicine, Fujita Health University, Toyoake 470-1192, Japan.

Division of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake 470-1192, Japan.

出版信息

J Clin Med. 2022 Aug 7;11(15):4603. doi: 10.3390/jcm11154603.

DOI:10.3390/jcm11154603

PMID:35956218

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9369602/

Abstract

BACKGROUND

is one of the significantly overexpressed genes found in a pre-eclamptic placenta and is involved with the etiology of this disease.

METHODS

We conducted genome-wide expression profiling by RNA-seq of placentas from women with pre-eclampsia and those with normotensive pregnancy.

RESULTS

We identified a lncRNA gene, , located ~80 kb upstream of the gene in a head-to-head orientation, which was overexpressed in the pre-eclamptic placenta. and are located within the same topologically associated domain in the genome. The mRNA level correlated with that of the in placentas from pre-eclamptic women as well as in samples from uncomplicated pregnancies. However, neither the overexpression nor knockdown of affected the expression of . Analysis of pre-eclampsia-linking genetic variants at this locus suggested that the placental genotype of one variant was associated with the expression of . The transcript level was not found to be associated with maternal blood pressure, but showed a relationship with birth and placental weights, suggesting that this lncRNA might be one of the pivotal placental factors in pre-eclampsia.

CONCLUSION

Further characterization of the gene may elucidate the etiological roles of the and genes in pre-eclampsia in a genomic context.

摘要

背景

是在子痫前期胎盘中发现的显著过表达基因之一，与该疾病的病因有关。

方法

我们通过RNA测序对患有子痫前期的女性和血压正常的孕妇的胎盘进行了全基因组表达谱分析。

结果

我们鉴定出一个lncRNA基因，其位于基因上游约80 kb处，呈头对头方向，在子痫前期胎盘中过表达。和位于基因组中同一个拓扑相关结构域内。子痫前期女性胎盘以及正常妊娠样本中，的mRNA水平与的mRNA水平相关。然而，的过表达或敲低均未影响的表达。对该位点与子痫前期相关的遗传变异分析表明，一个变异的胎盘基因型与的表达相关。未发现转录水平与孕妇血压相关，但与出生体重和胎盘重量有关，表明该lncRNA可能是子痫前期关键的胎盘因子之一。