Department of Mechanical and Aerospace Engineering, University of Central Florida, Orlando, FL 32816, USA.
Biionix Cluster, University of Central Florida, Orlando, FL 32827, USA.
Nutrients. 2022 Jul 31;14(15):3165. doi: 10.3390/nu14153165.
The influence of diet on the development of osteoporosis is significant and not fully understood. This study investigated the effect of diets of varying lipid profiles and ω-3, ω-6 and ω-9 composition on the structural and mechanical properties of bone. The hypothesis studied was that a diet high in saturated fat would induce osteoporosis and produce an overall increased detrimental bony response when compared with a diet high in unsaturated ω-6, or ω-9. Male C57BL/6J mice were fed either a control diet, 50:50 mix (saturated:unsaturated) high in ω-9 (HFD50:50), a diet high in saturated fat (HSF) or a polyunsaturated fat diet high in ω-6 (PUFA) over an 8-week duration. Tibiae were retrieved and evaluated using DMA, 3-point-bending, histomorphometry, and microCT. Mice fed a HSF diet displayed key features characteristic of osteoporosis. The loss tangent was significantly increased in the HFD50:50 diet group compared with control (p = 0.016) and PUFA-fed animals (p = 0.049). HFD50:50-fed mice presented with an increased viscous component, longer tibiae, increased loss modulus (p = 0.009), and ultimate stress, smaller microcracks (p < 0.001), and increased trabecular width (p = 0.002) compared with control animals. A diet high in ω-9 resulted in an overall superior bone response and further analysis of its role in bone health is warranted.
饮食对骨质疏松症的发展有重要影响，但尚未完全了解。本研究探讨了不同脂质谱和 ω-3、ω-6 和 ω-9 组成的饮食对骨骼结构和力学性能的影响。研究假设是，高脂肪饮食会导致骨质疏松症，并与富含 ω-6 或 ω-9 的不饱和饮食相比，产生整体增加的有害骨反应。雄性 C57BL/6J 小鼠分别用对照饮食、富含 ω-9 的 50:50 混合饮食（饱和：不饱和）（HFD50:50）、高脂肪饮食（HSF）或富含 ω-6 的多不饱和脂肪饮食（PUFA）喂养 8 周。使用 DMA、三点弯曲、组织形态计量学和 microCT 回收和评估胫骨。用 HSF 喂养的小鼠表现出骨质疏松症的典型特征。与对照组（p = 0.016）和 PUFA 喂养组（p = 0.049）相比，HFD50:50 饮食组的损耗因子显著增加。HFD50:50 喂养的小鼠具有更长的胫骨、增加的损耗模量（p = 0.009）和极限应力、更小的微裂纹（p < 0.001）和增加的小梁宽度（p = 0.002），与对照组相比。富含 ω-9 的饮食导致整体上更好的骨骼反应，需要进一步分析其在骨骼健康中的作用。
