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来自拟南芥的CH型锌指转录因子AtZAT4参与花粉和种子发育。

AtZAT4, a CH-Type Zinc Finger Transcription Factor from , Is Involved in Pollen and Seed Development.

作者信息

Puentes-Romero A Carolina, González Sebastián A, González-Villanueva Enrique, Figueroa Carlos R, Ruiz-Lara Simón

机构信息

Laboratorio de Genómica Funcional, Institute of Biological Sciences, Universidad de Talca, Talca 3460000, Chile.

Millenium Nucleus for the Development of Super Adaptable Plants (MN-SAP), Santiago 8340755, Chile.

出版信息

Plants (Basel). 2022 Jul 29;11(15):1974. doi: 10.3390/plants11151974.

DOI:10.3390/plants11151974
PMID:35956451
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9370812/
Abstract

Pollen plays an essential role in plant fertility by delivering the male gametes to the embryo sac before double fertilization. In several plant species, including Arabidopsis, C2H2-type zinc-finger transcription factors (TFs) have been involved in different stages of pollen development and maturation. ZINC FINGER of Arabidopsis thaliana 4 (AtZAT4) is homologous to such TFs and subcellular localization analysis has revealed that AtZAT4 is located in the nucleus. Moreover, analysis of AtZAT4 expression revealed strong levels of it in flowers and siliques, suggesting a role of the encoded protein in the regulation of genes that are associated with reproductive development. We characterized a T-DNA insertional heterozygous mutant Atzat4 (+/−). The relative gene expression analysis of Atzat4 (+/−) showed significant transcript reductions in flowers and siliques. Furthermore, the Atzat4 (+/−) phenotypic characterization revealed defects in the male germline, showing a reduction in pollen tube germination and elongation. Atzat4 (+/−) presented reduced fertility, characterized by a smaller silique size compared to the wild type (WT), and a lower number of seeds per silique. Additionally, seeds displayed lower viability and germination. Altogether, our data suggest a role for AtZAT4 in fertilization and seed viability, through the regulation of gene expression associated with reproductive development.

摘要

花粉在植物育性中起着至关重要的作用，它在双受精之前将雄配子传递到胚囊中。在包括拟南芥在内的几种植物物种中，C2H2型锌指转录因子（TFs）参与了花粉发育和成熟的不同阶段。拟南芥锌指蛋白4（AtZAT4）与这类转录因子同源，亚细胞定位分析表明AtZAT4定位于细胞核。此外，对AtZAT4表达的分析显示其在花和角果中有较高水平，这表明编码蛋白在调控与生殖发育相关的基因中发挥作用。我们鉴定了一个T-DNA插入杂合突变体Atzat4（+/-）。对Atzat4（+/-）的相对基因表达分析表明，其在花和角果中的转录本显著减少。此外，Atzat4（+/-）的表型特征显示雄性生殖系存在缺陷，表现为花粉管萌发和伸长减少。Atzat4（+/-）的育性降低，其特征是与野生型（WT）相比角果尺寸较小，每个角果中的种子数量较少。此外，种子的活力和发芽率较低。总之，我们的数据表明AtZAT4通过调控与生殖发育相关的基因表达，在受精和种子活力方面发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975f/9370812/300afb61a85d/plants-11-01974-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975f/9370812/60d778771514/plants-11-01974-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975f/9370812/c1961f58c12f/plants-11-01974-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975f/9370812/bff5d04b7cfa/plants-11-01974-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975f/9370812/1d180407323b/plants-11-01974-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975f/9370812/08a2afb56063/plants-11-01974-g005a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975f/9370812/300afb61a85d/plants-11-01974-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975f/9370812/60d778771514/plants-11-01974-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975f/9370812/c1961f58c12f/plants-11-01974-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975f/9370812/bff5d04b7cfa/plants-11-01974-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975f/9370812/1d180407323b/plants-11-01974-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975f/9370812/08a2afb56063/plants-11-01974-g005a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975f/9370812/300afb61a85d/plants-11-01974-g006a.jpg

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