Qin Tianyuan, Sun Chao, Kazim Ali, Cui Song, Wang Yihao, Richard Dormatey, Yao Panfeng, Bi Zhenzhen, Liu Yuhui, Bai Jiangping
Gansu Provincial Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.
National Institute for Genomics and Advanced Biotechnology, National Agricultural Research Centre, Park Road, Islamabad 45500, Pakistan.
Plants (Basel). 2022 Aug 3;11(15):2024. doi: 10.3390/plants11152024.
The selection and breeding of deep rooting and drought-tolerant varieties has become a promising approach for improving the yield and adaptability of potato ( L.) in arid and semiarid areas. Therefore, the discovery of root-development-related genes and drought tolerance signaling pathways in potato is important. In this study, we used deep-rooting (C119) and shallow-rooting (C16) potato genotypes, with different levels of drought tolerance, to achieve this objective. Both genotypes were treated with 150 mM mannitol for 0 h (T0), 2 h (T2), 6 h (T6), 12 h (T12), and 24 h (T24), and their root tissues were subjected to comparative transcriptome analysis. A total of 531, 1571, 1247, and 3540 differentially expressed genes (DEGs) in C16 and 1531, 1108, 674, and 4850 DEGs in C119 were identified in T2 vs. T0, T6 vs. T2, T12 vs. T6, and T24 vs. T12 comparisons, respectively. Gene expression analysis indicated that a delay in the onset of drought-induced transcriptional changes in C16 compared with C119. Functional enrichment analysis revealed genotype-specific biological processes involved in drought stress tolerance. The metabolic pathways of plant hormone transduction and MAPK signaling were heavily involved in the resistance of C16 and C119 to drought, while abscisic acid (ABA), ethylene, and salicylic acid signal transduction pathways likely played more important roles in C119 stress responses. Furthermore, genes involved in root cell elongation and division showed differential expression between the two genotypes under drought stress. Overall, this study provides important information for the marker-assisted selection and breeding of drought-tolerant potato genotypes.
选育深根耐旱品种已成为提高干旱和半干旱地区马铃薯产量及适应性的一种有前景的方法。因此，发现马铃薯中与根系发育相关的基因和耐旱信号通路很重要。在本研究中，我们使用了耐旱性不同的深根（C119）和浅根（C16）马铃薯基因型来实现这一目标。两种基因型均用150 mM甘露醇处理0小时（T0）、2小时（T2）、6小时（T6）、12小时（T12）和24小时（T24），并对其根组织进行比较转录组分析。在T2与T0、T6与T2、T12与T6以及T24与T12的比较中，分别在C16中鉴定出531、1571、1247和3540个差异表达基因（DEG），在C119中鉴定出1531、1108、674和4850个DEG。基因表达分析表明，与C119相比，C16中干旱诱导的转录变化起始延迟。功能富集分析揭示了参与耐旱性的基因型特异性生物学过程。植物激素转导和MAPK信号的代谢途径在C16和C119对干旱的抗性中起重要作用，而脱落酸（ABA）、乙烯和水杨酸信号转导途径可能在C119的胁迫反应中发挥更重要的作用。此外，参与根细胞伸长和分裂的基因在干旱胁迫下两种基因型之间表现出差异表达。总体而言，本研究为耐旱马铃薯基因型的分子标记辅助选择和育种提供了重要信息。
