Soria Federico, Aznar-Cervantes Salvador David, de la Cruz Julia E, Budia Alberto, Aranda Javier, Caballero Juan Pablo, Serrano Álvaro, Sánchez Margallo Francisco Miguel
Jesus Uson Minimally Invasive Surgery Centre Foundation, Endoscopy-Endourology Department, 10071 Cáceres, Spain.
Biotechnology Department, IMIDA, 30150 Murcia, Spain.
Polymers (Basel). 2022 Jul 28;14(15):3059. doi: 10.3390/polym14153059.
A major limitation of the treatment of low-grade upper tract urothelial carcinoma is the difficulty of intracavitary instillation of adjuvant therapy. Therefore, the aim of this in vitro study was to develop and to assess a new design of biodegradable ureteral stent coated with a silk fibroin matrix for the controlled release of mitomycin C as a chemotherapeutic drug. For this purpose, we assessed the coating of a biodegradable ureteral stent, BraidStent, with silk fibroin and subsequently loaded the polymeric matrix with two formulations of mitomycin to evaluate its degradation rate, the concentration of mitomycin released, and changes in the pH and the weight of the stent. Our results confirm that the silk fibroin matrix is able to coat the biodegradable stent and release mitomycin for between 6 and 12 h in the urinary environment. There was a significant delay in the degradation rate of silk fibroin and mitomycin-coated stents compared to bare biodegradable stents, from 6-7 weeks to 13-14 weeks. The present study has shown the feasibility of using mitomycin C-loaded silk fibroin for the coating of biodegradable urinary stents. The addition of mitomycin C to the coating of silk fibroin biodegradable stents could be an attractive approach for intracavitary instillation in the upper urinary tract.
低度上尿路尿路上皮癌治疗的一个主要局限是腔内灌注辅助治疗存在困难。因此，本体外研究的目的是研发并评估一种新型的可生物降解输尿管支架设计，该支架涂有丝素蛋白基质，用于控释化疗药物丝裂霉素C。为此，我们评估了用丝素蛋白对可生物降解输尿管支架BraidStent进行涂层，随后在聚合物基质中载入两种丝裂霉素制剂，以评估其降解率、丝裂霉素释放浓度以及支架的pH值和重量变化。我们的结果证实，丝素蛋白基质能够包覆可生物降解支架，并在尿液环境中6至12小时内释放丝裂霉素。与裸露的可生物降解支架相比，丝素蛋白和丝裂霉素涂层支架的降解率显著延迟，从6 - 7周延长至13 - 14周。本研究表明了使用载有丝裂霉素C的丝素蛋白包覆可生物降解尿路支架的可行性。在丝素蛋白可生物降解支架涂层中添加丝裂霉素C可能是上尿路腔内灌注的一种有吸引力的方法。
