Nuclear Medicine and Molecular Imaging Department, Institut de Cancérologie de Strasbourg Europe (ICANS), 17 Rue Albert Calmette, 67200 Strasbourg, France.
Institut Pluridisciplinaire Hubert Curien, Université de Strasbourg, 23 Rue du Loess, 67037 Strasbourg, France.
Molecules. 2022 Jul 29;27(15):4869. doi: 10.3390/molecules27154869.
CuCl is an economic radiotracer for oncologic PET investigations. In the present study, we characterized the uptake of CuCl in vivo by µPET/CT in an allograft 4T1-related mouse model (BALB/c) of advanced breast cancer. F-FDG was used as a comparator. Twenty-two animals were imaged 7-9 days following 4T1-cell implantation inside mammary glands. Dynamic CuCl µPET/CT acquisition or iterative static images up to 8 h p.i. were performed. Animal biodistribution and tumor uptake were first evaluated in vivo by µPET analysis and then assessed on tissue specimens. Concerning F-FDG µPET, a static acquisition was performed at 15 min and 60 min p.i. Tumor CuCl accumulation increased from 5 min to 4 h p.i., reaching a maximum value of 5.0 ± 0.20 %ID/g. Liver, brain, and muscle CuCl accumulation was stable over time. The tumor-to-muscle ratio remained stable from 1 to 8 h p.i., ranging from 3.0 to 3.7. Ex vivo data were consistent with in vivo estimations. The F-FDG tumor accumulation was 8.82 ± 1.03 %ID/g, and the tumor-to-muscle ratio was 4.54 ± 1.11. CuCl PET/CT provides good characterization of the 4T1-related breast cancer model and allows for exploration of non-glycolytic cellular pathways potentially of interest for theragnostic strategies.
CuCl 是一种经济的肿瘤正电子发射断层扫描（PET）放射性示踪剂。在本研究中，我们通过 µPET/CT 对 4T1 相关的晚期乳腺癌异体移植小鼠模型（BALB/c）中 CuCl 的体内摄取进行了特征描述。F-FDG 被用作比较剂。22 只动物在乳腺内植入 4T1 细胞后 7-9 天进行成像。进行了动态 CuCl µPET/CT 采集或迭代静态图像采集，直至注射后 8 小时。首先通过 µPET 分析对动物的生物分布和肿瘤摄取进行体内评估，然后在组织标本上进行评估。对于 F-FDG µPET，在注射后 15 分钟和 60 分钟进行静态采集。肿瘤 CuCl 积累从 5 分钟增加到 4 小时，达到 5.0±0.20%ID/g 的最大值。肝脏、大脑和肌肉中的 CuCl 积累随时间保持稳定。肿瘤与肌肉的比值在 1 至 8 小时内保持稳定，范围在 3.0 至 3.7 之间。体外数据与体内估计值一致。F-FDG 肿瘤积累为 8.82±1.03%ID/g，肿瘤与肌肉的比值为 4.54±1.11。CuCl PET/CT 可很好地对 4T1 相关的乳腺癌模型进行特征描述，并可探索可能对治疗策略有兴趣的非糖酵解细胞途径。
