School of Basic Medicine, Youjiang Medical University for Nationalities, Baise 533000, China.
Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, College of Life Science and Technology, Tarim University, Alar 843301, China.
Molecules. 2022 Jul 30;27(15):4881. doi: 10.3390/molecules27154881.
DC. was first recorded in the Chinese National Pharmacopoeia "Yi Plant Medicine". There is no detailed report on its main components' activity in suppressing the quorum sensing activity (QS) of bacteria. Our study aimed to screen the main components in extracts of DC. in order to measure their effects on bacterial QS activity and to explore specific quorum sensing mechanisms that are affected by DC. extracts. Crude extracts of DC. contained significant amounts of two compounds shown to inhibit bacterial QS activity, namely apigenin and luteolin. Apigenin and luteolin in crude extracts of DC. showed substantial inhibition of pigment formation, biofilm production, and motility in ATCC 12472 compared to the effects of other phytochemicals from DC. Apigenin and luteolin exhibited a strong QS inhibitory effect on , interfering with the violacein pigment biosynthesis by downregulating the , , and genes. In the presence of signal molecules, the QS effect is prevented, and the selected compounds can still inhibit the production of the characteristic purple pigment in . Based on qualitative and quantitative research using genomics and bioinformatics, we concluded that apigenin and luteolin in crude extracts of DC can interfere with the generation of QS in by downregulating the , , and genes. Indeed, DC. is used for the treatment of bacterial infections, and this research provides new ideas and potential alternative uses for medicinal plants.
穿心莲最早被记录在中国国家药典《一草志》中。关于其主要成分抑制细菌群体感应活性（QS）的活性，尚无详细报道。本研究旨在筛选穿心莲提取物中的主要成分，以测量它们对细菌 QS 活性的影响，并探索受穿心莲提取物影响的特定群体感应机制。穿心莲的粗提物中含有两种被证明能抑制细菌 QS 活性的化合物，即芹菜素和木樨草素。与穿心莲其他植物化学物质相比，粗提物中的芹菜素和木樨草素能显著抑制 ATCC 12472 中色素形成、生物膜生成和运动性。芹菜素和木樨草素对 QS 表现出强烈的抑制作用，通过下调 、 、 和 基因干扰了紫色素生物合成。在信号分子存在的情况下，QS 效应被阻止，所选化合物仍能抑制 中特征性紫色色素的产生。基于基因组学和生物信息学的定性和定量研究，我们得出结论，穿心莲粗提物中的芹菜素和木樨草素可以通过下调 、 、 和 基因来干扰 中 QS 的产生。事实上，穿心莲被用于治疗细菌感染，这项研究为药用植物提供了新的思路和潜在的替代用途。
