Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Tarim University, Alaer, P. R. China.
Engineering Laboratory of Chemical Resources Utilization in South Xinjiang of Xinjiang Production and Construction Corps, Tarim University, Alaer, P. R. China.
Nat Prod Res. 2021 Dec;35(23):5508-5512. doi: 10.1080/14786419.2020.1788554. Epub 2020 Jul 8.
In our study, the anti-quorum sensing (QS) activity of fermentation broth from TRM B-02, a bacterium isolated from Taklimakan desert, was investigated using the biosensor bioassay on ATCC12472. TRM B-02 was 100% similar to subsp. Inaquosorum KCTC 13429 by genotypic and phenotypic analyses. Based on anti-QS activity tracking, six known amicoumacins, named as AI-77-H (), AI-77-F (), amicoumacin B (), amicoumacin C (), AI-77-C () and bacilosarcins D (), were isolated and identified. Among them, compounds exhibited a better inhibitory effect on ATCC12472. Further research suggested that compounds - could significantly down-regulate the expressions of violacein operon A (vioA), vioB, vioD and vioE and up-regulate vioC. Docking experiments indicated that compounds may act as an inhibitor of violacein biosynthetic pathway competitively inhibiting the binding of flavin adenine dinucleotide (FAD) with the vioD enzyme.[Figure: see text].
在我们的研究中，使用生物传感器生物测定法在 ATCC12472 上研究了从塔里木沙漠分离的细菌 TRM B-02 的发酵液的抗群体感应 (QS) 活性。TRM B-02 通过基因型和表型分析与 subsp. Inaquosorum KCTC 13429 100%相似。基于抗 QS 活性跟踪，分离并鉴定了六种已知的amicoumacins，命名为 AI-77-H (), AI-77-F (), amicoumacin B (), amicoumacin C (), AI-77-C () 和 bacilosarcins D (). 其中，化合物 - 对 ATCC12472 表现出更好的抑制作用。进一步的研究表明，化合物 - 可显著下调 violacein 操纵子 A (vioA)、vioB、vioD 和 vioE 的表达，并上调 vioC。对接实验表明，化合物 - 可能作为 violacein 生物合成途径的抑制剂，竞争性抑制黄素腺嘌呤二核苷酸 (FAD) 与 vioD 酶的结合。[图：见正文]。
