Institute of Quality Standards and Testing Technology for Agricultural Products, Chinese Academy of Agricultural Science, Beijing 100081, China.
Institute of Quality Standards and Testing Technology for Agricultural Products, Xinjiang Academy of Agricultural Science, Urumqi 830091, China.
Molecules. 2022 Aug 4;27(15):4953. doi: 10.3390/molecules27154953.
An ionic liquid-modified molecularly imprinted polymer (IL-MIP) composite with sulfamethazine as a template molecule and methyl acrylic acid and 1-aminopropyl-3-methylimidazolium bromide as functional monomers was successfully synthesized. The achieved IL-MIP was characterized and evaluated in detail and utilized in the extraction and cleanup of sulfonamides (SAs) in poultry egg samples. The results demonstrated that the IL-MIP possessed a broad reorganization toward SAs and could selectively adsorb 21 kinds of SA compounds. Furthermore, the solid-phase extraction column based on the IL-MIP was used in the extraction and cleanup of 21 SAs in eggs, and the confirmatory detection of SAs was performed using ultraperformance liquid chromatography−tandem mass spectrometry. Under optimum conditions, the limits of detection (LODs) for all SAs ranged from 0.1 ng·g−1 to 1.5 ng·g−1, and the LOD of this method was better than those of the existing methods. The recoveries of SA compounds spiked in egg samples ranged from 84.3% to 105.8%, with low relative standard deviations (<15%). The developed method based on the IL-MIP extraction and cleanup was successfully used in the detection of 21 SAs in more than 100 real poultry egg samples. The results indicated that the proposed method was suitable for detecting 21 SAs in poultry eggs.
以磺胺甲噁唑为模板分子，甲基丙烯酸和 1-氨基丙基-3-甲基咪唑溴盐为功能单体，成功合成了一种离子液体修饰的分子印迹聚合物（IL-MIP）复合材料。详细表征和评价了所得到的 IL-MIP，并将其用于禽蛋样品中磺胺类药物（SAs）的提取和净化。结果表明，IL-MIP 对 SAs 具有广泛的重排作用，并能选择性地吸附 21 种 SA 化合物。此外，基于 IL-MIP 的固相萃取柱用于鸡蛋中 21 种 SA 的提取和净化，并采用超高效液相色谱-串联质谱法对 SA 进行确证检测。在最佳条件下，所有 SA 的检测限（LOD）范围为 0.1 ng·g−1至 1.5 ng·g−1，该方法的 LOD 优于现有方法。在鸡蛋样品中添加的 SA 化合物的回收率在 84.3%至 105.8%之间，相对标准偏差较低（<15%）。基于 IL-MIP 提取和净化的开发方法成功用于检测 100 多个实际禽蛋样品中的 21 种 SA。结果表明，该方法适用于检测禽蛋中的 21 种 SA。
