Department of Chemical Engineering, Nanchang University, Nanchang 330031, China.
School of Foreign Language, Nanchang University, Nanchang 330031, China.
Molecules. 2022 Oct 27;27(21):7304. doi: 10.3390/molecules27217304.
In order to improve the mechanical strength and imprinting efficiency, a novel bovine serum albumin (BSA) molecularly imprinted poly(ionic liquid)/calcium alginate composite cryogel membrane (MICM) was prepared. The results of the tensile test indicated that the MICM had excellent mechanical strength which could reach up to 90.00 KPa, 30.30 times higher than the poly (ionic liquid) membrane without calcium alginate; the elongation of it could reach up to 93.70%, 8.28 times higher than the poly (ionic liquid) membrane without calcium alginate. The MICM had a very high welling ratio of 1026.56% and macropore porosity of 62.29%, which can provide effective mass transport of proteins. More remarkably, it had a very high adsorption capacity of 485.87 mg g at 20 °C and 0.66 mg mL of the initial concentration of BSA. Moreover, MICM also had good selective and competitive recognition toward BSA, exhibiting potential utility in protein separation. This work can provide a potential method to prepare the protein-imprinted cryogel membrane with both high mechanical strength and imprinting efficiency.
为了提高机械强度和印迹效率，制备了一种新型牛血清白蛋白（BSA）分子印迹聚离子液体/海藻酸钠复合冷冻凝胶膜（MICM）。拉伸试验结果表明，MICM 具有优异的机械强度，可达 90.00 KPa，比不含海藻酸钠的聚离子液体膜高 30.30 倍；其伸长率可达 93.70%，比不含海藻酸钠的聚离子液体膜高 8.28 倍。MICM 的溶胀率高达 1026.56%，大孔孔隙率为 62.29%，可为蛋白质的有效传质提供条件。更值得注意的是，它在 20°C 和初始 BSA 浓度为 0.66mg/mL 时的吸附容量高达 485.87mg/g。此外，MICM 对 BSA 还具有良好的选择性和竞争性识别，在蛋白质分离方面具有潜在的应用价值。这项工作为制备具有高机械强度和印迹效率的蛋白质印迹冷冻凝胶膜提供了一种潜在的方法。
