College of Chemistry and Materials Science, Shanghai Normal University, Shanghai 200234, China.
State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
Molecules. 2022 Aug 7;27(15):5020. doi: 10.3390/molecules27155020.
Ultralong hydroxyapatite (HAP) nanowires are promising for various biomedical applications owing to their chemical similarity to the inorganic constituent of bone, high biocompatibility, good flexibility, excellent mechanical properties, etc. However, it is still challenging to control the formation of ultralong HAP nanowires because of the presence of free PO ions in the reaction system containing the inorganic phosphate source. In addition, it takes a long period of time (usually tens of hours) for the synthetic process of ultralong HAP nanowires. Herein, for the first time, we have developed an eco-friendly calcium oleate precursor microwave hydrothermal method using biocompatible adenosine 5'-triphosphate (ATP) as a bio-phosphorus source and water as the only solvent for the rapid synthesis of ultralong HAP nanowires. The controllable hydrolysis of ATP can avoid the premature formation of calcium phosphate nuclei and uncontrollable crystal growth. Microwave heating can significantly shorten the synthetic time from tens of hours required by the traditional heating to 1 h, thus achieving high efficiency, energy saving and low cost. The as-prepared ultralong HAP nanowires with high flexibility have lengths of several hundred micrometers and diameters of 10~20 nm, and they usually self-assemble into nanowire bundles along their longitudinal direction. The as-prepared ultralong HAP nanowire/chitosan porous scaffold has excellent bioactivity, good biodegradation and cytocompatibility owing to the bioactive adenosine adsorbed on the surface of ultralong HAP nanowires. It is expected that ultralong HAP nanowires will be promising for various applications in the biomedical fields, such as bone defect repair, skin wound healing, and as a drug nanocarrier.
超长羟基磷灰石（HAP）纳米线由于其与骨无机成分的化学相似性、高生物相容性、良好的柔韧性、优异的机械性能等，在各种生物医学应用中具有广阔的前景。然而，由于含有无机磷酸盐源的反应体系中存在游离的 PO43-离子，控制超长 HAP 纳米线的形成仍然具有挑战性。此外，超长 HAP 纳米线的合成过程需要很长的时间（通常需要数十个小时）。在此，我们首次开发了一种使用生物相容性的腺苷 5'-三磷酸（ATP）作为生物磷源、水作为唯一溶剂的环保型油酸钠钙前体微波水热法，用于快速合成超长 HAP 纳米线。ATP 的可控水解可以避免磷酸钙核的过早形成和不可控的晶体生长。微波加热可以显著缩短从传统加热所需的数十小时的合成时间至 1 小时，从而实现高效、节能和低成本。所制备的超长 HAP 纳米线具有柔韧性，长度可达数百微米，直径为 10~20nm，通常沿其纵向自组装成纳米线束。所制备的超长 HAP 纳米线/壳聚糖多孔支架具有良好的生物活性、良好的生物降解性和细胞相容性，这是由于表面吸附的具有生物活性的腺苷。预计超长 HAP 纳米线将在骨缺损修复、皮肤伤口愈合以及作为药物纳米载体等生物医学领域的各种应用中具有广阔的前景。
