NanoBioMedical Centre, Adam Mickiewicz University in Poznań, Wszechnicy Piastowskiej 3, 61-614 Poznań, Poland.
NanoBioMedical Centre, Adam Mickiewicz University in Poznań, Wszechnicy Piastowskiej 3, 61-614 Poznań, Poland.
Mater Sci Eng C Mater Biol Appl. 2021 May;124:112062. doi: 10.1016/j.msec.2021.112062. Epub 2021 Mar 26.
This work aimed to study for the first time to our knowledge the influence of the structure of the dental flosses (DF) coated by hydroxyapatite nanoparticles (HAP NPs) on the biological performance of saliva probiotic bacteria (S. salivarius), and human dermal and osteoblast-like cells. We used three types of HAP@DF composites (based on two unwaxed dental flosses - "fluffy" and "smooth", and one waxed "smooth") with different morphologies. Obtained composites were characterized from the point of view of their structure, morphological characteristics, elemental and chemical composition. We observed that HAP NPs coated "smooth" dental flosses led to an increase of viability and proliferation of oral cavity probiotic bacteria (Streptococcus salivarius) and human cells (dermal fibroblasts and osteoblast-like). In contrast, the highest viability loss of probiotic bacteria (S. salivarius), fibroblasts, and osteoblast-like cells were observed for "fluffy" unwaxed dental flosses due to high cytotoxicity. Our studies showed that HAP NPs significantly improved the biological properties of "fluffy" dental floss. Pristine "smooth" DFs (waxed and unwaxed), as well as all HAP-coated DFs, induced acceptable biocompatibility toward selected human cells.
本研究首次考察了经纳米羟基磷灰石（HAP NPs）涂层处理的牙线结构对唾液益生菌（S. salivarius）以及人真皮成纤维细胞和骨样细胞生物学性能的影响。我们使用了三种类型的 HAP@DF 复合材料（基于两种未上蜡的牙线——“蓬松”和“光滑”，以及一种上蜡的“光滑”牙线），它们具有不同的形态。从结构、形态特征、元素和化学组成的角度对所得复合材料进行了表征。我们观察到，HAP NPs 涂层“光滑”牙线可提高口腔益生菌（唾液链球菌）和人细胞（真皮成纤维细胞和骨样细胞）的活力和增殖。相比之下，由于高细胞毒性，蓬松的未上蜡牙线导致益生菌（S. salivarius）、成纤维细胞和骨样细胞活力下降最明显。我们的研究表明，HAP NPs 可显著改善蓬松牙线的生物学性能。原始的“光滑”牙线（上蜡和未上蜡）以及所有 HAP 涂层牙线对选定的人细胞均表现出可接受的生物相容性。
