Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Electronic Science, Xiamen University, Xiamen 361005, China.
Molecules. 2022 Aug 8;27(15):5032. doi: 10.3390/molecules27155032.
The micellar solubilization mechanism of curcumin by mixed surfactants of SDS and Brij35 was investigated at the molecular scale by NMR spectroscopy. Through the investigation of the micelle formation process, types and structures of mixed micelles and solubilization sites, the intrinsic factors influencing the solubilization capacity were revealed. For systems with α = 0.5 and 0.2, the obtained molar solubilization ratios (MSRs) are consistent with the MSR values. However, for α = 0.8, the solubilization capacity of curcumin is weakened compared to the MSR. Furthermore, only one single mixed SDS/Brij35 micelles are formed for α = 0.5 and 0.2. However, for α = 0.8, there are separate SDS-rich and Brij35-rich mixed micelles formed. In addition, NOESY spectra show that the interaction patterns of SDS and Brij35 in mixed micelles are similar for three systems, as are the solubilization sites of curcumin. Therefore, for α = 0.5 and 0.2 with single mixed micelles formed, the solubility of curcumin depends only on the mixed micelle composition, which is almost equal to the surfactant molar ratio. Although curcumin is solubilized in both separate micelles at α = 0.8, a less stable micelle structure may be responsible for the low solubility. This study provides new insights into the investigation and application of mixed micelle solubilization.
通过 NMR 光谱研究了 SDS 和 Brij35 混合表面活性剂对姜黄素的胶束增溶机制。通过考察胶束形成过程、混合胶束的类型和结构以及增溶位置，揭示了影响增溶能力的内在因素。对于α=0.5 和 0.2 的体系，得到的摩尔增溶比（MSR）与 MSR 值一致。然而，对于α=0.8 的体系，与 MSR 相比，姜黄素的增溶能力减弱。此外，对于α=0.5 和 0.2，只形成了单一的 SDS/Brij35 混合胶束。然而，对于α=0.8，形成了分离的 SDS 富和 Brij35 富混合胶束。此外，NOESY 谱表明，三种体系中 SDS 和 Brij35 在混合胶束中的相互作用模式相似，姜黄素的增溶位置也相似。因此，对于形成单一混合胶束的α=0.5 和 0.2，姜黄素的溶解度仅取决于混合胶束的组成，这几乎等于表面活性剂的摩尔比。尽管在α=0.8 时姜黄素分别溶解在两个独立的胶束中，但可能是不太稳定的胶束结构导致溶解度较低。本研究为混合胶束增溶的研究和应用提供了新的见解。
