Kong Lingdan, Zhu Jingyi, Su Hongxing, Zhao Lingzhou, Lu Yi, Zhu Meilin, Sun Wenjie
School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, China.
School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing, China.
Front Bioeng Biotechnol. 2022 Jul 22;10:973141. doi: 10.3389/fbioe.2022.973141. eCollection 2022.
We report a new I-labeling functional platform for targeted single-photon emission computed tomography (SPECT) imaging and radiotherapy of breast adenocarcinoma. In this study, polyethyleneimine (PEI) based nanogels (P.NH NGs) were prepared by water/oil polymerization, modified with targeted agent phenylboronic acid (PBA), and labeled with radionuclide I. The NGs without I-labeling own a spherical structure, uniform size distribution, and good cell viability. After I-labeling, the obtained I-PBA-PHP NGs displayed much higher cellular uptake than the non-targeted NGs due to the good softness and fluidity of NGs and the PBA targeting. The results demonstrated that I-PBA-PHP NGs could specifically target breast cancer cells and efficiently aggregate into xenograft breast adenocarcinoma for tumor SPECT imaging and specific radiotherapy. The developed I-labeling NGs may be used as a promising platform for efficient radioactive theranostic nanoplatform of tumor.
我们报道了一种用于乳腺腺癌靶向单光子发射计算机断层扫描（SPECT）成像和放射治疗的新型碘标记功能平台。在本研究中，通过水/油聚合制备了基于聚乙烯亚胺（PEI）的纳米凝胶（P.NH NGs），用靶向剂苯硼酸（PBA）进行修饰，并用放射性核素碘进行标记。未进行碘标记的纳米凝胶具有球形结构、均匀的尺寸分布和良好的细胞活力。碘标记后，由于纳米凝胶良好的柔软性和流动性以及PBA靶向作用，所获得的碘-苯硼酸-聚（β-氨基酯）纳米凝胶（I-PBA-PHP NGs）表现出比非靶向纳米凝胶更高的细胞摄取量。结果表明，I-PBA-PHP NGs可以特异性靶向乳腺癌细胞，并有效地聚集到异种移植乳腺腺癌中用于肿瘤SPECT成像和特异性放射治疗。所开发的碘标记纳米凝胶可能作为一种有前景的肿瘤高效放射性诊疗纳米平台。
