Pallavicini M G, Levin J, Summers L, Levin F
Exp Hematol. 1987 Jul;15(6):704-9.
Multivariate flow cytometric analysis and sorting of mouse bone marrow cells viably stained with Hoechst 33342 (HO) was utilized to obtain a subpopulation enriched at least 30- to 60-fold for clonogenic megakaryocyte precursors (CFU-Meg). HO fluorescence intensity, forward light scatter, and perpendicular light scatter were measured on each HO-stained bone marrow cell as it passed through a dual beam flow cytometer. Cells were sorted on the basis of their light scattering and HO fluorescence intensity properties. The sorting region which included cells with high forward light scatter, low perpendicular light scatter, and low (0.1-0.65 times the modal channel) HO fluorescence intensity contained approximately 0.6% CFU-Meg. Of all CFU-Meg, 75% were contained in the light scatter gate, and 56% of all CFU-Meg were in the low HO-sorting region. This technique offers a simple, one-step procedure to obtain preparations enriched in murine clonogenic megakaryocyte precursors.
利用多参数流式细胞术对用Hoechst 33342（HO）进行活细胞染色的小鼠骨髓细胞进行分析和分选，以获得富含克隆形成性巨核细胞前体（CFU-Meg）至少30至60倍的亚群。当每个经HO染色的骨髓细胞通过双光束流式细胞仪时，测量其HO荧光强度、前向光散射和侧向光散射。根据细胞的光散射和HO荧光强度特性进行分选。分选区域包括前向光散射高、侧向光散射低且HO荧光强度低（模式通道的0.1 - 0.65倍）的细胞，其中约含0.6%的CFU-Meg。在所有CFU-Meg中，75%位于光散射门内，56%位于低HO分选区域。该技术提供了一种简单的一步法程序，以获得富含小鼠克隆形成性巨核细胞前体的制剂。
