Jackson H, Williams N, Bertoncello I, Green R
Department of Physiology, University of Melbourne, Parkville, Victoria, Australia.
Exp Hematol. 1994 Sep;22(10):954-8.
We describe high proliferative potential colony-forming cells-megakaryocyte (HPP-CFU-Mk) from mouse bone marrow preparations using known cytokine combinations. These primitive precursors, which generate at least 80 megakaryocytes per colony, were detected from bone marrow populations enriched for primitive cells, either following treatment with 5-fluorouracil (5-FU) or after enrichment from normal bone marrow using immunological procedures. HPP-CFU-Mk were most reproducibly grown in the presence of interleukin-1 (IL-1) plus IL-3 plus IL-6, and mostly grew as a single large aggregate, rarely forming multiple foci. Cell separation studies showed that the HPP-CFU-Mk have membrane properties that characterized these cells as being intermediate between high proliferative myeloid primitive progenitors (high proliferative colony-forming cells [HPP-CFC]) and committed megakaryocyte progenitors (CFU-Mk). The data show that HPP-CFU-Mk can be designated as primitive cells on the basis of their being spared in vivo after 5-FU treatment, their proliferative potential to produce megakaryocytes, and their cofractionation with a proportion of primitive myeloid progenitor cells.
我们使用已知的细胞因子组合，从小鼠骨髓制备物中描述了高增殖潜能集落形成细胞-巨核细胞（HPP-CFU-Mk）。这些原始前体细胞每个集落可产生至少80个巨核细胞，可从经5-氟尿嘧啶（5-FU）处理后富集原始细胞的骨髓群体中检测到，或使用免疫学方法从正常骨髓中富集后检测到。HPP-CFU-Mk在白细胞介素-1（IL-1）加IL-3加IL-6存在的情况下生长最为稳定，且大多以单个大聚集体形式生长，很少形成多个集落。细胞分离研究表明，HPP-CFU-Mk具有膜特性，这些特性将这些细胞表征为介于高增殖性髓系原始祖细胞（高增殖集落形成细胞[HPP-CFC]）和定向巨核细胞祖细胞（CFU-Mk）之间。数据表明，基于5-FU处理后在体内得以保留、产生巨核细胞的增殖潜能以及与一部分原始髓系祖细胞的共分级分离，HPP-CFU-Mk可被指定为原始细胞。
