Radithia Desiana, Soebadi Bagus, Parmadiati Adiastuti Endah, Winias Saka
Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
J Oral Biol Craniofac Res. 2022 Sep-Oct;12(5):570-574. doi: 10.1016/j.jobcr.2022.07.006. Epub 2022 Jul 19.
Chronic orofacial pain is associated with nerve tissues damage. Pharmacological therapy has limited therapeutic results because it is generally only symptomatic treatment. Neuroregeneration is a process which is needed to repair damaged of nerve tissue through healing or regrowth of nerve tissue. The survival of nerve cells need neurotrophic factors including Nerve Growth Factor (NGF) and S100B. High platelet concentrations in Platelet Rich Plasma contain of many trophic factors which play an important role in peripheral nerve regeneration following nerve injury. The aim of the present study is to analyze the increased expression of NGF and S100B following injection of Freeze-Dried Platelet Rich Plasma (FD-PRP) on axonotmesis injury.
Fifty-four male wistar rats aged 3 months randomly divided into 3 groups; negative control group (without nerve injury and without FD-PRP injection), positive control group (nerve injury but without FD-PRP injection) and treatment group (nerve injury and FD-PRP injection). Axonotmesis nerve injury created by clamping the infraorbital nerve for 15 s. Application of FD-PRP by injection technique. Examination of NGF and S100B expression was obtained by immunohistochemistry examination with monoclonal antibodies (anti-NGF and anti-S100B). Samples were taken on the 14th day and 21st day.
Treatment group showed significant increase on both NGF and S100B compare to positive control (p = 0,000 and p = 0,000, respectively).
FD-PRP injection is effective in inducing neuroregeneration by increasing NGF and S100B expression.
慢性口面部疼痛与神经组织损伤有关。药物治疗的效果有限，因为它通常只是对症治疗。神经再生是一个通过神经组织的愈合或再生来修复受损神经组织的过程。神经细胞的存活需要神经营养因子，包括神经生长因子（NGF）和S100B。富血小板血浆中高浓度的血小板含有许多营养因子，它们在神经损伤后的周围神经再生中起重要作用。本研究的目的是分析冻干富血小板血浆（FD-PRP）注射后轴突断裂损伤中NGF和S100B表达的增加情况。
将54只3个月大的雄性Wistar大鼠随机分为3组；阴性对照组（无神经损伤且未注射FD-PRP）、阳性对照组（有神经损伤但未注射FD-PRP）和治疗组（有神经损伤且注射FD-PRP）。通过夹闭眶下神经15秒造成轴突断裂性神经损伤。采用注射技术应用FD-PRP。通过使用单克隆抗体（抗NGF和抗S100B）的免疫组织化学检查来检测NGF和S100B的表达。在第14天和第21天取样。
与阳性对照组相比，治疗组的NGF和S100B均显著增加（分别为p = 0,000和p = 0,000）。
FD-PRP注射通过增加NGF和S100B的表达有效诱导神经再生。
