Winias Saka, Sarasati Andari, Wicaksono Satutya, Ayuningtyas Nurina Febriyanti, Ernawati Diah Savitri, Radithia Desiana
Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Eur J Dent. 2024 Feb;18(1):131-137. doi: 10.1055/s-0043-1761194. Epub 2023 Jun 13.
Various growth factors contained in PRP can increase angiogenesis and cell proliferation, which plays an essential role in the process of neuroregeneration and peripheral nerve injury recovery. This study analyzed PRP effects in the neuro-regeneration of axonotmesis through brain-derived neurotrophic factor (BDNF) and Krox20 expressions.
Freeze-dried allogeneic platelet-rich plasma (PRP) were prepared from allogeneic sources. Forty-two were divided into three groups: negative control group, positive control group (crushing infraorbital nerve) and treatment group (crushing infraorbital nerve without PRP injection). Each group was observed for fourteen and twenty-one days after injury. Infraorbital nerve tissue is isolated for indirect immunohistochemistry examination with BDNF and Krox20 antibodies. Data analysis was performed using One-Way ANOVA and Mann-Whitney tests with significant value as p < 0.05.
The PRP group showed BDNF expression significantly higher than control positive groups, both observation days (p = 0.00). A higher Korx20 expression showed by the PRP group after 21 days than in the control positive groups (p = 0.002).
PRP can potentially improve neuroregeneration of axonotmesis through increased BDNF and Krox20 expression on the twenty-one days after injury.
富血小板血浆（PRP）中含有的多种生长因子可促进血管生成和细胞增殖，这在神经再生和周围神经损伤恢复过程中起着至关重要的作用。本研究通过脑源性神经营养因子（BDNF）和Krox20表达分析PRP对轴突断裂神经再生的影响。
从异体来源制备冻干异体富血小板血浆（PRP）。42只（实验对象未明确，推测是动物）分为三组：阴性对照组、阳性对照组（眶下神经挤压伤）和治疗组（眶下神经挤压伤但未注射PRP）。每组在损伤后14天和21天进行观察。分离眶下神经组织，用BDNF和Krox20抗体进行间接免疫组织化学检查。采用单因素方差分析和Mann-Whitney检验进行数据分析，以p < 0.05为有统计学意义。
在两个观察日，PRP组的BDNF表达均显著高于阳性对照组（p = 0.00）。PRP组在21天后的Krox20表达高于阳性对照组（p = 0.002）。
PRP可能通过在损伤后21天增加BDNF和Krox20表达来改善轴突断裂的神经再生。
