Creasy D M, Beech L M, Gray T J, Butler W H
Exp Mol Pathol. 1987 Jun;46(3):357-71. doi: 10.1016/0014-4800(87)90056-6.
A sequential morphological study has been carried out to examine the ultrastructural effects of di-n-pentyl phthalate (DPP) on the mature rat testis. A single oral dose of 2.2 g DPP/kg body wt was administered, and testes, perfuse-fixed 3-48 hr after dosing, were examined by transmission electron microscopy. By 3 hr, rarefaction of the basal Sertoli cell cytoplasm was seen and the basal plasma membranes separating adjacent Sertoli cells were thrown into a series of convoluted profiles with the appearance of interdigitating cell processes. The subjacent ectoplasmic specializations that normally face these membranes were disrupted, and by 12 hr the inter-Sertoli junctions showed numerous membrane discontinuities. The lateral processes of Sertoli cell cytoplasm, which separate germ cells, showed retraction and fragmentation, resulting in direct contact between adjacent germ cells or the isolation of germ cells unapposed by Sertoli cell plasma membrane. In addition, the ectoplasmic specializations associated with Sertoli-spermatid and Sertoli-pachytene spermatocyte junctions were often disrupted or absent. The mitochondria in the Sertoli cells were enlarged and, in some tubules, increased in number. The changes seen were restricted to tubules in the successive stages XI-XIV, I, and II of the spermatogenic cycle. Elongating spermatids (steps 12-15) showed cytoplasmic condensation and vacuolation by 12 hr and were necrotic by 24 hr. A small proportion of zygotene and early pachytene spermatocytes showed necrosis by 24 hr after dosing. By 48 hr, the cytoplasmic rarefaction and convoluted plasma membranes had regressed and ectroplasmic specializations had reformed along Sertoli-Sertoli junctions.
已进行了一项连续性形态学研究，以检查邻苯二甲酸二正戊酯（DPP）对成年大鼠睾丸的超微结构影响。给予单次口服剂量为2.2 g DPP/kg体重，给药后3 - 48小时进行灌注固定，然后通过透射电子显微镜检查睾丸。3小时时，可见支持细胞基底细胞质出现稀疏，分隔相邻支持细胞的基底质膜形成一系列曲折的轮廓，呈现出细胞相互交错的突起外观。通常面对这些膜的下方胞质特化结构被破坏，到12小时时，支持细胞间连接显示出许多膜间断。分隔生殖细胞的支持细胞细胞质侧突出现回缩和断裂，导致相邻生殖细胞直接接触或生殖细胞未被支持细胞质膜覆盖而孤立。此外，与支持细胞 - 精子细胞和支持细胞 - 粗线期精母细胞连接相关的胞质特化结构常常被破坏或缺失。支持细胞中的线粒体增大，在一些小管中数量也增加。所观察到的变化仅限于生精周期连续阶段XI - XIV、I和II的小管。伸长的精子细胞（第12 - 15步）在12小时时出现细胞质浓缩和空泡化，24小时时坏死。一小部分偶线期和早期粗线期精母细胞在给药后24小时出现坏死。到48小时时，细胞质稀疏和曲折的质膜已消退，支持细胞 - 支持细胞连接处的胞质特化结构已重新形成。
