Creasy D M, Foster J R, Foster P M
J Pathol. 1983 Mar;139(3):309-21. doi: 10.1002/path.1711390307.
Prepubertal rats treated orally with di-n-pentyl phthalate at 2.2 g/kg body weight were killed at 1, 3, 6 and 24 hr following a single dose, and after 2, 3 and 4 days of repeated daily dosing. At 3 hr Sertoli cells in a proportion of the seminiferous tubules showed vacuolation of the perinuclear smooth endoplasmic reticulum with an associated inward displacement of germinal cells. By 6 hr the vacuolation had extended to the apical cytoplasm and was evident in most tubules. Early degenerative changes were also apparent in spermatocytes and spermatids and were accompanied by an acute interstitial inflammatory infiltrate. By 24 hr, germinal cell degeneration was extensive with desquamation and general disorganisation of cell layers within the epithelium, but the interstitial inflammatory infiltrate had declined. Mitochondrial succinic dehydrogenase activity in Sertoli cells was reduced at 3 and 6 hr and absent by 24 hr. In germinal cells it was unaffected at 3 and 6 hr but absent by 24 hr. Two, three and four days of daily phthalate treatment resulted in a gradual depletion of germinal cells from all tubules, leaving a Sertoli cell matrix containing a few necrotic spermatocytes and occasional normal spermatogonia. The significance of the early Sertoli cell changes is discussed.
给青春期前大鼠按2.2克/千克体重口服邻苯二甲酸二正戊酯，单次给药后1、3、6和24小时以及每日重复给药2、3和4天后将其处死。3小时时，部分生精小管中的支持细胞显示核周滑面内质网空泡化，同时生殖细胞向内移位。到6小时时，空泡化已扩展至顶端细胞质，且在大多数小管中明显可见。精母细胞和精子细胞也出现早期退行性变化，并伴有急性间质炎性浸润。到24小时时，生殖细胞变性广泛，上皮内细胞层出现脱落和普遍紊乱，但间质炎性浸润已减轻。支持细胞中的线粒体琥珀酸脱氢酶活性在3和6小时时降低，到24小时时消失。在生殖细胞中，该活性在3和6小时时未受影响，但到24小时时消失。连续2、3和4天给予邻苯二甲酸酯导致所有小管中的生殖细胞逐渐耗竭，留下一个支持细胞基质，其中含有一些坏死的精母细胞和偶尔的正常精原细胞。文中讨论了早期支持细胞变化的意义。
