Yuan Shan, Wang Yining, Wang Junya, Zhang Chunlei, Zhang Lixin, Jiang Bingjun, Wu Tingting, Chen Li, Xu Xin, Cai Yupeng, Sun Shi, Chen Fulu, Song Wenwen, Wu Cunxiang, Hou Wensheng, Yu Lijie, Han Tianfu
MARA Key Laboratory of Soybean Biology (Beijing), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
College of Life Science and Technology, Harbin Normal University, Harbin, China.
Front Plant Sci. 2022 Jul 25;13:929747. doi: 10.3389/fpls.2022.929747. eCollection 2022.
Onset of flowering of plants is precisely controlled by extensive environmental factors and internal molecular networks, in which () is a key flowering integrator. In soybean, a typical short-day plant, 11 homologues are found in its genome, of which several homologues are functionally diversified in flowering pathways and the others including are yet unknown. In the current study, we characterized , which is located on the same chromosome as the flowering promoters and . Overexpression of significantly promoted flowering of Arabidopsis under the inductive long-day (LD) photoperiod. over-expressed soybean also flowered earlier than the control under LD, but they were not significantly different under inductive short-day (SD) conditions, indicating that acts as a flowering promoter in the non-inductive photoperiod in soybean. Compared with other homologues, exhibited a slighter effect in flowering promotion than , and under LD conditions. promoted flowering by regulating the expression of downstream flowering-related genes and also affected the expression of other . According to the re-sequencing data, the regional distributions of two major haplotypes in 176 soybean varieties were analyzed. The varieties with -Hap2 haplotype matured relatively early, and relative higher expression of was detected in early maturing varieties, implying that Hap2 variation may contribute to the adaptation of soybean to higher latitude regions by increasing expression level of genes in metabolism and signaling pathways. The early flowering germplasm generated by overexpression of has potential to be planted at higher latitudes where non-inductive long day is dominant in the growing season, and can be used to fine-tune soybean flowering and maturity time and improve the geographical adaptation.
植物开花的起始受到广泛的环境因素和内部分子网络的精确调控，其中（）是关键的开花整合因子。在典型的短日植物大豆中，其基因组中发现了11个同源物，其中几个同源物在开花途径中功能多样，而其他包括（此处原文缺失信息）在内的同源物功能尚不清楚。在本研究中，我们对（此处原文缺失信息）进行了表征，它与开花促进因子（此处原文缺失信息）和（此处原文缺失信息）位于同一条染色体上。在诱导长日（LD）光周期下，（此处原文缺失信息）的过表达显著促进了拟南芥的开花。过表达（此处原文缺失信息）的大豆在长日条件下也比对照更早开花，但在诱导短日（SD）条件下它们之间没有显著差异，这表明（此处原文缺失信息）在大豆的非诱导光周期中作为开花促进因子起作用。与其他（此处原文缺失信息）同源物相比，在长日条件下，（此处原文缺失信息）在促进开花方面的作用比（此处原文缺失信息）、（此处原文缺失信息）和（此处原文缺失信息）更微弱。（此处原文缺失信息）通过调节下游开花相关基因的表达来促进开花，并且还影响其他（此处原文缺失信息）的表达。根据重测序数据，分析了176个大豆品种中两种主要单倍型的区域分布。具有 -Hap2单倍型的品种成熟相对较早，并且在早熟品种中检测到相对较高的（此处原文缺失信息）表达，这意味着Hap2变异可能通过增加代谢和信号通路中基因的表达水平来促进大豆对高纬度地区的适应。通过过表达（此处原文缺失信息）产生的早花种质有潜力种植在生长季节以非诱导长日为主的高纬度地区，并且（此处原文缺失信息）可用于微调大豆的开花和成熟时间并改善地理适应性。
