大鼠脑中表达的蛋白激酶C多种亚型结构的鉴定。

Identification of the structures of multiple subspecies of protein kinase C expressed in rat brain.

作者信息

Kikkawa U, Ono Y, Ogita K, Fujii T, Asaoka Y, Sekiguchi K, Kosaka Y, Igarashi K, Nishizuka Y

出版信息

FEBS Lett. 1987 Jun 15;217(2):227-31. doi: 10.1016/0014-5793(87)80668-3.

Abstract

Rat brain protein kinase C purified to apparent homogeneity [(1986) Biochem. Biophys. Res. Commun. 135, 636-643] was resolved into three distinct fractions, type I, II and III, upon chromatography on a hydroxyapatite column connected to high-performance liquid chromatography. Comparison of each fraction with the four subspecies of protein kinase C, that were separately expressed in COS cells transfected by the respective cDNAs, alpha, beta I, beta II and d gamma, identified the primary structures of these three fractions of protein kinase C. Type I corresponded to the enzyme encoded by the gamma-sequence; type II was a mixture of the two subspecies determined by the beta I- and beta II-sequences; and type III had the structure encoded by the alpha-sequence. The structures and properties of these subspecies of protein kinase C were similar to each other.

摘要

纯化至表观均一的大鼠脑蛋白激酶C[（1986年）《生物化学与生物物理学研究通讯》135卷，636 - 643页]在连接到高效液相色谱的羟基磷灰石柱上进行色谱分离时，被分离为三个不同的组分，即I型、II型和III型。将每个组分与分别在由相应cDNA（α、βI、βII和δγ）转染的COS细胞中单独表达的蛋白激酶C的四个亚型进行比较，确定了蛋白激酶C这三个组分的一级结构。I型对应于由γ序列编码的酶；II型是由βI和βII序列决定的两个亚型的混合物；III型具有由α序列编码的结构。这些蛋白激酶C亚型的结构和性质彼此相似。