Ohsugi M, Yamamura H, Semba R, Hidaka H
Department of Anatomy, Mie University School of Medicine, Japan.
Histochem J. 1994 Aug;26(8):641-3. doi: 10.1007/BF00158288.
To confirm the possibility that protein kinase C is involved in compaction of mouse embryos, the presence and distribution pattern of Ca(2+)-dependent subspecies of this enzyme in mouse embryos, before and during compaction, were examined immunocytochemically with three different monoclonal antibodies. These were MC-1a, MC-2a and MC-3a, which selectively interact with the subspecies of the enzyme known as types I, II and III, respectively. Only when embryos were incubated with MC-3a, was immunofluorescence clearly detected in all cells of embryos before and during compaction. This result demonstrates the presence of type III protein kinase C in embryos before and during compaction and suggests the possibility that the type III enzyme may be involved in compaction. No marked differences were found in the distribution pattern of the type III enzyme between embryos examined before and during compaction.
为了证实蛋白激酶C参与小鼠胚胎致密化的可能性，利用三种不同的单克隆抗体通过免疫细胞化学方法检测了该酶的钙依赖性亚型在小鼠胚胎致密化之前及致密化过程中的存在情况和分布模式。这三种抗体分别是MC-1a、MC-2a和MC-3a，它们分别与该酶的I型、II型和III型亚型选择性相互作用。只有当胚胎与MC-3a一起孵育时，在致密化之前及致密化过程中胚胎的所有细胞中才能清楚地检测到免疫荧光。这一结果证明了III型蛋白激酶C在胚胎致密化之前及致密化过程中的存在，并提示III型酶可能参与致密化的可能性。在致密化之前和致密化过程中检测的胚胎之间，III型酶的分布模式没有发现明显差异。
