α型和β型蛋白激酶C诱导通透化垂体细胞中的胞吐作用。
Induction of exocytosis in permeabilized pituitary cells by alpha- and beta-type protein kinase C.
作者信息
Naor Z, Dan-Cohen H, Hermon J, Limor R
机构信息
Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, Israel.
出版信息
Proc Natl Acad Sci U S A. 1989 Jun;86(12):4501-4. doi: 10.1073/pnas.86.12.4501.
Abstract
Protein kinase C is now recognized to comprise a family of closely related subspecies (PKCs). When cultured rat pituitary cells were permeabilized by digitonin for 5 min in the absence of Ca2+, endogenous PKC activity was decreased by 72%. PKC depletion was also achieved by prior treatment (24 hr) with high concentrations of phorbol 12-myristate 13-acetate (PMA). When purified activated brain PKCs were added for 30 min to PMA-pretreated, digitonin-permeabilized cells, only alpha- and beta- but not gamma-type PKC stimulated luteinizing hormone release. Since PKC was implicated as a mediator of gonadotropin secretion, gonadotropin-releasing hormone might utilize alpha- and beta-type PKCs for stimulation of gonadotropin secretion; alpha- and beta-type PKCs might participate also in other exocytotic responses in diverse biological systems in which PKC was implicated.
摘要
蛋白激酶C现在被认为是由一系列密切相关的亚类（PKC）组成。当在无Ca2+的情况下，用洋地黄皂苷将培养的大鼠垂体细胞通透处理5分钟时，内源性PKC活性降低了72%。通过预先用高浓度佛波酯12 -肉豆蔻酸酯13 -乙酸酯（PMA）处理（24小时）也能实现PKC的耗竭。当将纯化的活化脑PKC添加到经PMA预处理、洋地黄皂苷通透处理的细胞中30分钟时，只有α型和β型而非γ型PKC刺激促黄体生成素释放。由于PKC被认为是促性腺激素分泌的介质，促性腺激素释放激素可能利用α型和β型PKC来刺激促性腺激素分泌；α型和β型PKC也可能参与PKC涉及的多种生物系统中的其他胞吐反应。
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