Lorenzo J L, Allorio M, Bernini F, Corsini A, Fumagalli R
FEBS Lett. 1987 Jun 22;218(1):77-80. doi: 10.1016/0014-5793(87)81022-0.
Oxygenated derivatives of cholesterol are believed to play a role in cellular cholesterol homeostasis through the feed-back control of its biosynthesis. We report that 26-hydroxycholesterol inhibits the specific binding, uptake and degradation of 125I-LDL in human fibroblasts. The effect is dose-dependent, and saturation kinetics indicates a reduction of LDL-binding sites with no effect on ligand affinity. The results support a possible role of 26-hydroxycholesterol, a physiological oxysterol, in the regulation of cellular cholesterol homeostasis.
胆固醇的氧化衍生物被认为通过对其生物合成的反馈控制在细胞胆固醇稳态中发挥作用。我们报告，26-羟基胆固醇抑制人成纤维细胞中125I-LDL的特异性结合、摄取和降解。该效应呈剂量依赖性，饱和动力学表明LDL结合位点减少，而对配体亲和力无影响。这些结果支持了生理氧化甾醇26-羟基胆固醇在调节细胞胆固醇稳态中可能发挥的作用。
