Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Department of Orthopedics, Guanghua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.
J Immunol Res. 2022 Aug 1;2022:9634073. doi: 10.1155/2022/9634073. eCollection 2022.
The mechanisms underlying osteoarthritis (OA) have recently been hypothesized to involve a dysfunctional immune system. In this study, we collected synovium, synovial fluid (SF), and peripheral blood from 21 patients. Mononuclear cells were characterized using FCM. H&E staining and mIHC histological assessment of synovium were performed. Cytokine levels in the SF were measured using ELISA. We observed similar frequencies of immune cells in the synovium and SF, which were enriched in DCs. Notably, CD1cCD163 DC3s were expanded in the synovium and SF. Furthermore, we found that DC3s were primarily located within the ectopic lymphoid-like structure (ELLS) in close proximity to CD8 T cells. Finally, the level of TNF- and IL12p70 in the SF correlated with the severity of OA. These data suggest that OA is an immune system-related disease and that DC3s may play an active role in OA progression by promoting ELLS formation and inflammatory responses.
骨关节炎（OA）的发病机制最近被假设涉及免疫系统功能障碍。在这项研究中，我们收集了 21 名患者的滑膜、滑液（SF）和外周血。使用 FCM 对单核细胞进行了特征描述。对滑膜进行了 H&E 染色和 mIHC 组织学评估。使用 ELISA 测量了 SF 中的细胞因子水平。我们观察到滑膜和 SF 中的免疫细胞频率相似，这些细胞富含 DC。值得注意的是，CD1cCD163 DC3 在滑膜和 SF 中扩增。此外，我们发现 DC3 主要位于与 CD8 T 细胞紧邻的异位淋巴样结构（ELLS）内。最后，SF 中的 TNF-和 IL12p70 水平与 OA 的严重程度相关。这些数据表明 OA 是一种与免疫系统相关的疾病，并且 DC3 可能通过促进 ELLS 形成和炎症反应而在 OA 进展中发挥积极作用。
