Yao Xin, Wang Teng, Sun Meng Yang, Yuming Yang, Guixin Duan, Liu Jing
Medical College of Nantong University, Nantong, China.
Department of Bioinformatics, School of Basic Medical Sciences, Chongqing Medical University, Chongqing, China.
Biomarkers. 2022 Sep;27(6):526-533. doi: 10.1080/1354750X.2022.2085799. Epub 2022 Aug 12.
Since the role of long non-coding RNA (lncRNA) HOTAIR is yet to be established in non-small cell lung cancer (NSCLC), we tried to explore the expression of lncRNA HOTAIR in NSCLC and evaluate the correlation between the combined detection of lncRNA HOTAIR and routine tumour markers and the pathological staging of lung cancer.This study prospectively included 148 patients with NSCLC selected from our hospital from January 2017 to September 2020 as the lung cancer group, and 148 healthy volunteers who referred for physical examination were selected as the control group. Fluorescence in situ hybridisation was used to detect the expression of lncRNA HOTAIR in the cancerous tissues and adjacent tissues of lung cancer patients; the immunofluorescence method was used to detect the serum NSE, CEA and CYFRA21-1 levels of the two groups of testers. Correlation analysis was used to evaluate any relation between cancer staging and markers. In addition, ROC curve analysis was used to estimate sensitivity, specificity, positive predictive value, and negative predictive value.The expression of lncRNA HOTAIR in lung cancer tissues was higher than control or surrounding tissue (<0.05). Also, high levels of NSE, CEA and CYFRA21-1 were observed in lung cancer group (<0.05). In both N and T stage, the expression of lncRNA HOTAIR combined with NSE, CEA and CYFRA21-1 levels increased with the increase in the number of stages (<0.05). The results of single factor analysis showed that NSE, CEA, CYFRA21-1 and lncRNA HOTAIR all have appropriate diagnostic value for detecting lung cancer (specificity of 92.6, 91.5, 90.6, 86.9%, respectively and the sensitivity of 61.3, 62.9, 55.4, 52.3%, respectively).LncRNA HOTAIR is a novel diagnostic test with high diagnostic value for detecting of pathological staging of NSCLC; however, the diagnostic accuracy of lncRNA HOTAIR is not higher than other tumour biomarkers.
由于长链非编码RNA（lncRNA）HOTAIR在非小细胞肺癌（NSCLC）中的作用尚未明确，我们试图探究lncRNA HOTAIR在NSCLC中的表达情况，并评估lncRNA HOTAIR与常规肿瘤标志物联合检测和肺癌病理分期之间的相关性。本研究前瞻性纳入了2017年1月至2020年9月期间我院收治的148例NSCLC患者作为肺癌组，选取148例因体检前来就诊的健康志愿者作为对照组。采用荧光原位杂交法检测肺癌患者癌组织及癌旁组织中lncRNA HOTAIR的表达；采用免疫荧光法检测两组检测者血清中的NSE、CEA和CYFRA21-1水平。采用相关性分析评估癌症分期与标志物之间的关系。此外，采用ROC曲线分析来估计敏感性、特异性、阳性预测值和阴性预测值。lncRNA HOTAIR在肺癌组织中的表达高于对照组或周围组织（<0.05）。此外，肺癌组中NSE、CEA和CYFRA21-1水平较高（<0.05）。在N期和T期，lncRNA HOTAIR联合NSE、CEA和CYFRA21-1水平均随着分期数的增加而升高（<0.05）。单因素分析结果显示，NSE、CEA、CYFRA21-1和lncRNA HOTAIR对肺癌检测均具有一定的诊断价值（特异性分别为92.6%、91.5%、90.6%、86.9%，敏感性分别为61.3%、62.9%、55.4%、52.3%）。lncRNA HOTAIR是一种对NSCLC病理分期检测具有较高诊断价值的新型诊断方法；然而，lncRNA HOTAIR的诊断准确性并不高于其他肿瘤生物标志物。
